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dc.contributor.authorMartinerie, Laetitiaen_US
dc.contributor.authorManterola, Marciaen_US
dc.contributor.authorChung, Sanny S. W.en_US
dc.contributor.authorPanigrahi, Sunil K.en_US
dc.contributor.authorWeisbach, Melissaen_US
dc.contributor.authorVasileva, Anaen_US
dc.contributor.authorGeng, Yanen_US
dc.contributor.authorSicinski, Peteren_US
dc.contributor.authorWolgemuth, Debra J.en_US
dc.date.accessioned2014-04-11T14:13:05Z
dc.date.issued2014en_US
dc.identifier.citationMartinerie, Laetitia, Marcia Manterola, Sanny S. W. Chung, Sunil K. Panigrahi, Melissa Weisbach, Ana Vasileva, Yan Geng, Peter Sicinski, and Debra J. Wolgemuth. 2014. “Mammalian E-type Cyclins Control Chromosome Pairing, Telomere Stability and CDK2 Localization in Male Meiosis.” PLoS Genetics 10 (2): e1004165. doi:10.1371/journal.pgen.1004165. http://dx.doi.org/10.1371/journal.pgen.1004165.en
dc.identifier.issn1553-7390en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12064534
dc.description.abstractLoss of function of cyclin E1 or E2, important regulators of the mitotic cell cycle, yields viable mice, but E2-deficient males display reduced fertility. To elucidate the role of E-type cyclins during spermatogenesis, we characterized their expression patterns and produced additional deletions of Ccne1 and Ccne2 alleles in the germline, revealing unexpected meiotic functions. While Ccne2 mRNA and protein are abundantly expressed in spermatocytes, Ccne1 mRNA is present but its protein is detected only at low levels. However, abundant levels of cyclin E1 protein are detected in spermatocytes deficient in cyclin E2 protein. Additional depletion of E-type cyclins in the germline resulted in increasingly enhanced spermatogenic abnormalities and corresponding decreased fertility and loss of germ cells by apoptosis. Profound meiotic defects were observed in spermatocytes, including abnormal pairing and synapsis of homologous chromosomes, heterologous chromosome associations, unrepaired double-strand DNA breaks, disruptions in telomeric structure and defects in cyclin-dependent-kinase 2 localization. These results highlight a new role for E-type cyclins as important regulators of male meiosis.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pgen.1004165en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937215/pdf/en
dash.licenseLAAen_US
dc.subjectBiologyen
dc.subjectDevelopmental Biologyen
dc.subjectCell Differentiationen
dc.subjectFertilizationen
dc.subjectGeneticsen
dc.subjectAnimal Geneticsen
dc.subjectCytogeneticsen
dc.subjectGene Functionen
dc.subjectGenetics of Diseaseen
dc.subjectMolecular Geneticsen
dc.subjectGenomicsen
dc.subjectChromosome Biologyen
dc.subjectChromatinen
dc.subjectChromosome Structure and Functionen
dc.subjectMeiosisen
dc.subjectMitosisen
dc.subjectTelomeresen
dc.subjectStructural Genomicsen
dc.subjectHistologyen
dc.subjectMolecular Cell Biologyen
dc.subjectCell Divisionen
dc.subjectCyclinsen
dc.subjectCellular Structuresen
dc.subjectCell Nucleusen
dc.subjectCellular Typesen
dc.subjectGerm Cellsen
dc.titleMammalian E-type Cyclins Control Chromosome Pairing, Telomere Stability and CDK2 Localization in Male Meiosisen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Geneticsen
dash.depositing.authorGeng, Yanen_US
dc.date.available2014-04-11T14:13:05Z
dc.identifier.doi10.1371/journal.pgen.1004165*
dash.contributor.affiliatedGeng, Yan


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