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dc.contributor.authorMaeder, Morgan Len_US
dc.contributor.authorLinder, Samantha Jen_US
dc.contributor.authorCascio, Vincent Men_US
dc.contributor.authorFu, Yanfangen_US
dc.contributor.authorHo, Quan Hen_US
dc.contributor.authorJoung, J Keithen_US
dc.date.accessioned2014-05-06T16:15:50Z
dc.date.issued2013en_US
dc.identifier.citationMaeder, Morgan L, Samantha J Linder, Vincent M Cascio, Yanfang Fu, Quan H Ho, and J Keith Joung. 2013. “CRISPR RNA-guided activation of endogenous human genes.” Nature methods 10 (10): 977-979. doi:10.1038/nmeth.2598. http://dx.doi.org/10.1038/nmeth.2598.en
dc.identifier.issn1548-7091en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12152832
dc.description.abstractCatalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation domain fusion protein can be directed by single or multiple gRNAs to increase expression of specific endogenous human genes. These results provide an important proof-of-principle that CRISPR-Cas systems can be used to target heterologous effector domains in human cells.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nmeth.2598en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794058/pdf/en
dash.licenseLAAen_US
dc.titleCRISPR RNA-guided activation of endogenous human genesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature methodsen
dash.depositing.authorLinder, Samantha Jen_US
dc.date.available2014-05-06T16:15:50Z
dc.identifier.doi10.1038/nmeth.2598*
dash.contributor.affiliatedFu, Yanfang
dash.contributor.affiliatedLinder, Sam


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