Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development
Inlay, Matthew A.
Fathman, John W.
Dimov, Ivan K.
Weissman, Irving L.
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CitationInlay, Matthew A., Thomas Serwold, Adriane Mosley, John W. Fathman, Ivan K. Dimov, Jun Seita, and Irving L. Weissman. 2014. “Identification of Multipotent Progenitors that Emerge Prior to Hematopoietic Stem Cells in Embryonic Development.” Stem Cell Reports 2 (4): 457-472. doi:10.1016/j.stemcr.2014.02.001. http://dx.doi.org/10.1016/j.stemcr.2014.02.001.
AbstractSummary Hematopoiesis in the embryo proceeds in a series of waves, with primitive erythroid-biased waves succeeded by definitive waves, within which the properties of hematopoietic stem cells (multilineage potential, self-renewal, and engraftability) gradually arise. Whereas self-renewal and engraftability have previously been examined in the embryo, multipotency has not been thoroughly addressed, especially at the single-cell level or within well-defined populations. To identify when and where clonal multilineage potential arises during embryogenesis, we developed a single-cell multipotency assay. We find that, during the initiation of definitive hematopoiesis in the embryo, a defined population of multipotent, engraftable progenitors emerges that is much more abundant within the yolk sac (YS) than the aorta-gonad-mesonephros (AGM) or fetal liver. These experiments indicate that multipotent cells appear in concert within both the YS and AGM and strongly implicate YS-derived progenitors as contributors to definitive hematopoiesis.
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