Show simple item record

dc.contributor.authorOrtiz-Meoz, Rodrigo F.en_US
dc.contributor.authorMerbl, Yifaten_US
dc.contributor.authorKirschner, Marc W.en_US
dc.contributor.authorWalker, Suzanneen_US
dc.date.accessioned2014-05-06T16:17:59Z
dc.date.issued2014en_US
dc.identifier.citationOrtiz-Meoz, Rodrigo F., Yifat Merbl, Marc W. Kirschner, and Suzanne Walker. 2014. “Microarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylated.” Journal of the American Chemical Society 136 (13): 4845-4848. doi:10.1021/ja500451w. http://dx.doi.org/10.1021/ja500451w.en
dc.identifier.issn0002-7863en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12152955
dc.description.abstractO-GlcNAc transferase (OGT) is a serine/threonine glycosyltransferase that is essential for development and continues to be critically important throughout life. Understanding OGT’s complex biology requires identifying its substrates. Here we demonstrate the utility of a microarray approach for discovering novel OGT substrates. We also report a rapid method to validate OGT substrates that combines in vitro transcription-translation with O-GlcNAc mass tagging. Among the validated new OGT targets is Orthodenticle homeobox 2 (OTX2), a transcription factor critical for brain development, which is primarily expressed only during early embryogenesis and in medulloblastomas, where it functions as an oncogene. We show that endogenous OTX2 from a medulloblastoma cell line is O-GlcNAcylated at several sites. Our results demonstrate that protein microarray technology, combined with the target validation strategy we report, is useful for identifying biologically important OGT substrates, including substrates not present in most tissue types or cell lines.en
dc.language.isoen_USen
dc.publisherAmerican Chemical Societyen
dc.relation.isversionofdoi:10.1021/ja500451wen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988687/pdf/en
dash.licenseLAAen_US
dc.titleMicroarray Discovery of New OGT Substrates: The Medulloblastoma Oncogene OTX2 Is O-GlcNAcylateden
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of the American Chemical Societyen
dash.depositing.authorMerbl, Yifaten_US
dc.date.available2014-05-06T16:17:59Z
dc.identifier.doi10.1021/ja500451w*
dash.contributor.affiliatedMerbl, Yifat Haya
dash.contributor.affiliatedKirschner, Marc


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record