The potential dual effects of sevoflurane on AKT/GSK3β signaling pathway

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The potential dual effects of sevoflurane on AKT/GSK3β signaling pathway

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Title: The potential dual effects of sevoflurane on AKT/GSK3β signaling pathway
Author: Zhang, Lei; Zhang, Jie; Dong, Yuanlin; Swain, Celeste A; Zhang, Yiying; Xie, Zhongcong

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Citation: Zhang, Lei, Jie Zhang, Yuanlin Dong, Celeste A Swain, Yiying Zhang, and Zhongcong Xie. 2014. “The potential dual effects of sevoflurane on AKT/GSK3β signaling pathway.” Medical Gas Research 4 (1): 5. doi:10.1186/2045-9912-4-5. http://dx.doi.org/10.1186/2045-9912-4-5.
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Abstract: Background: Anesthesia with multiple exposures of commonly used inhalation anesthetic sevoflurane induces neuroinflammation and cognitive impairment in young mice, but anesthesia with a single exposure to sevoflurane does not. AKT/glycogen synthase kinase 3β (GSK3β) signaling pathway is involved in neurotoxicity and neurobehavioral deficits. However, whether sevoflurane can induce a dual effect (increase versus decrease) on the activation of AKT/GSK3β signaling pathway remains to be determined. We therefore set out to assess the effects of sevoflurane on AKT/GSK3β signaling pathway in vivo and in vitro. Methods: Six day-old wild-type mice were exposed to 3% sevoflurane two hours daily for one or three days. In the in vitro studies, H4 human neuroglioma cells were treated with 4% sevoflurane for two or six hours. We then determined the effects of different sevoflurane treatments on the levels of phosphorylated (P)-GSK3β(ser9) and P-AKT(ser473) by using Western blot analysis. Results: Here we show that anesthesia with 3% sevoflurane two hours daily for one day increased the levels of P-GSK3β(ser9) and P-AKT(ser473), but the anesthesia with 3% sevoflurane daily for three days decreased them in the mice. The treatment with 4% sevoflurane for two hours increased, but the treatment with 4% sevoflurane for six hours decreased, the levels of P-GSK3β(ser9) and P-AKT(ser473) in the H4 human neuroglioma cells. Conclusions: Anesthetic sevoflurane might induce a dual effect (increase versus decrease) on the activation of the AKT/GSK3β signaling pathway. These studies have established a system to perform further studies to determine the effects of sevoflurane on brain function.
Published Version: doi:10.1186/2045-9912-4-5
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996018/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12152992
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