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dc.contributor.advisorChurch, George McDonald
dc.contributor.authorHuang, Po-Yi
dc.date.accessioned2014-06-06T16:34:09Z
dash.embargo.terms2015-06-04en_US
dc.date.issued2014-06-06
dc.date.submitted2014
dc.identifier.citationHuang, Po-Yi. 2014. Study on Bacterial Protein Synthesis System toward the Incorporation of D-Amino Acid & Synthesis of 2'-deoxy-3'-mercapto-tRNA. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:11365en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12274132
dc.description.abstractLife is anti-entropic and highly organized phenomenon with two characteristics reinforcing each other: homochirality and the stereospecific catalysis of chemical reactions. The exclusive presence of L-amino acids and R-sugars in living world well depict this. Hypothetically, the amino acids and sugars of reverse chirality could form a parallel kingdom which is highly orthogonal to the present world. The components from this mirror kingdom, such as protein or nucleic acid, will be much more resistant to the defensive mechanism of present living system, which could be of great value. Therefore, by gradually rewiring the present bio-machineries, we look to build a bridge leading us to the space of mirror-imaged biomolecules. We begin by investigating protein synthesis with mirror amino acid since most amino acids contain one chiral center to be inversed comparing to sugars. In this work, we analyzed three stages critical for the incorporation of D-amino acid into ribosomal protein synthesis: amino acylation, EF-Tu binding of amino acyl-tRNA and delivery bias, and ribosome catalyzed peptidyl transfer. We have demonstrated that the affinity between EF-Tu and amino acyl-tRNA plays critical role on D-amino acid incorporation, and built a platform aimed to select for ribosome tolerating D-amino acid better.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dash.licenseLAA
dc.subjectBiochemistryen_US
dc.subjectD-amino aciden_US
dc.subjectEF-Tuen_US
dc.subjectRibosome engineeringen_US
dc.titleStudy on Bacterial Protein Synthesis System toward the Incorporation of D-Amino Acid & Synthesis of 2'-deoxy-3'-mercapto-tRNAen_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorHuang, Po-Yi
dc.date.available2017-04-22T07:31:38Z
thesis.degree.date2014en_US
thesis.degree.disciplineChemistry and Chemical Biologyen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberChurch, Georgeen_US
dc.contributor.committeeMemberLiu, Daviden_US
dc.contributor.committeeMemberSzostak, Jacken_US
dash.contributor.affiliatedHuang, Po-Yi


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