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dc.contributor.advisorZon, Leonard Ira
dc.contributor.authorGanis, Jared Jason
dc.date.accessioned2014-06-07T01:26:12Z
dash.embargo.terms2015-06-04en_US
dash.embargo.terms2015-06-04
dc.date.issued2014-06-06
dc.date.submitted2014
dc.identifier.citationGanis, Jared Jason. 2014. Regulators of hemoglobin switching in zebrafish and human models. Doctoral dissertation, Harvard University.en_US
dc.identifier.otherhttp://dissertations.umi.com/gsas.harvard:11391en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12274627
dc.description.abstractHemoglobin switching is a developmental process involving the dynamic transcriptional regulation of multiple globin genes. This molecular process involves multiple layer of complexity, and elucidating new mechanisms in this process will result in a more complete understanding of general gene regulation and will likely have direct clinical implications for hemoglobinopathies, such as sickle cell anemia. In this dissertation, I develop and characterize a new model for hemoglobin switching, the zebrafish. I defined and fully annotated the two zebrafish globin loci, termed major and minor loci. Both loci contain α– and β–genes oriented in a head–to–head fashion. Characterization of the globin expression pattern precisely defined the timing of normal switching and demonstrated that zebrafish, like humans, have two globin switches. The locus control region for the major locus was identified and in conjunction with a proximal promoter was able to generate robust, erythroid–specific expression in a transgenic line.en_US
dc.language.isoen_USen_US
dash.licenseLAA
dc.subjectDevelopmental biologyen_US
dc.subjectglobinen_US
dc.subjecthemogobinen_US
dc.subjectswitchingen_US
dc.subjectzebrafishen_US
dc.titleRegulators of hemoglobin switching in zebrafish and human modelsen_US
dc.typeThesis or Dissertationen_US
dash.depositing.authorGanis, Jared Jason
dc.date.available2015-06-04T07:30:50Z
thesis.degree.date2014en_US
thesis.degree.disciplineBiology: Medical Sciences, Division ofen_US
thesis.degree.grantorHarvard Universityen_US
thesis.degree.leveldoctoralen_US
thesis.degree.namePh.D.en_US
dc.contributor.committeeMemberCantor, Alanen_US
dc.contributor.committeeMemberDaley, Georgeen_US
dc.contributor.committeeMemberLowrey, Christopheren_US
dash.contributor.affiliatedGanis, Jared Jason


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