Show simple item record

dc.contributor.authorChia, Joanne S.J.en_US
dc.contributor.authorMcRae, Jennifer L.en_US
dc.contributor.authorThomas, Helen E.en_US
dc.contributor.authorFynch, Staceyen_US
dc.contributor.authorElkerbout, Lorraineen_US
dc.contributor.authorHill, Prueen_US
dc.contributor.authorMurray-Segal, Lisaen_US
dc.contributor.authorRobson, Simon C.en_US
dc.contributor.authorChen, Jiang-Fanen_US
dc.contributor.authord’Apice, Anthony J.F.en_US
dc.contributor.authorCowan, Peter J.en_US
dc.contributor.authorDwyer, Karen M.en_US
dc.date.accessioned2014-07-07T17:03:00Z
dc.date.issued2013en_US
dc.identifier.citationChia, J. S., J. L. McRae, H. E. Thomas, S. Fynch, L. Elkerbout, P. Hill, L. Murray-Segal, et al. 2013. “The Protective Effects of CD39 Overexpression in Multiple Low-Dose Streptozotocin–Induced Diabetes in Mice.” Diabetes 62 (6): 2026-2035. doi:10.2337/db12-0625. http://dx.doi.org/10.2337/db12-0625.en
dc.identifier.issn0012-1797en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12406646
dc.description.abstractIslet allograft survival limits the long-term success of islet transplantation as a potential curative therapy for type 1 diabetes. A number of factors compromise islet survival, including recurrent diabetes. We investigated whether CD39, an ectonucleotidase that promotes the generation of extracellular adenosine, would mitigate diabetes in the T cell–mediated multiple low-dose streptozotocin (MLDS) model. Mice null for CD39 (CD39KO), wild-type mice (WT), and mice overexpressing CD39 (CD39TG) were subjected to MLDS. Adoptive transfer experiments were performed to delineate the efficacy of tissue-restricted overexpression of CD39. The role of adenosine signaling was examined using mutant mice and pharmacological inhibition. The susceptibility to MLDS-induced diabetes was influenced by the level of expression of CD39. CD39KO mice developed diabetes more rapidly and with higher frequency than WT mice. In contrast, CD39TG mice were protected. CD39 overexpression conferred protection through the activation of adenosine 2A receptor and adenosine 2B receptor. Adoptive transfer experiments indicated that tissue-restricted overexpression of CD39 conferred robust protection, suggesting that this may be a useful strategy to protect islet grafts from T cell–mediated injury.en
dc.language.isoen_USen
dc.publisherAmerican Diabetes Associationen
dc.relation.isversionofdoi:10.2337/db12-0625en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661652/pdf/en
dash.licenseLAAen_US
dc.subjectIslet Studiesen
dc.titleThe Protective Effects of CD39 Overexpression in Multiple Low-Dose Streptozotocin–Induced Diabetes in Miceen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalDiabetesen
dash.depositing.authorRobson, Simon C.en_US
dc.date.available2014-07-07T17:03:00Z
dc.identifier.doi10.2337/db12-0625*
dash.authorsorderedfalse
dash.contributor.affiliatedRobson, Simon


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record