The Microglial Sensome Revealed by Direct RNA Sequencing

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The Microglial Sensome Revealed by Direct RNA Sequencing

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Title: The Microglial Sensome Revealed by Direct RNA Sequencing
Author: Hickman, Suzanne E.; Kingery, Nathan D.; Ohsumi, Toshiro; Borowsky, Mark; Wang, Li-chong; Means, Terry K.; Khoury, Joseph El

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Citation: Hickman, Suzanne E., Nathan D. Kingery, Toshiro Ohsumi, Mark Borowsky, Li-chong Wang, Terry K. Means, and Joseph El Khoury. 2013. “The Microglial Sensome Revealed by Direct RNA Sequencing.” Nature neuroscience 16 (12): 10.1038/nn.3554. doi:10.1038/nn.3554.
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Abstract: Microglia, the principal neuroimmune sentinels of the brain, continuously sense changes in their environment and respond to invading pathogens, toxins and cellular debris. Microglia exhibit plasticity and can assume neurotoxic or neuroprotective priming states that determine their responses to danger. We used direct RNA sequencing, without amplification or cDNA synthesis, to determine the quantitative transcriptomes of microglia of healthy adult and aged mice. We validated our findings by fluorescent dual in-situ hybridization, unbiased proteomic analysis and quantitative PCR. We report here that microglia have a distinct transcriptomic signature and express a unique cluster of transcripts encoding proteins for sensing endogenous ligands and microbes that we term the “sensome”. With aging, sensome transcripts for endogenous ligand recognition are downregulated, whereas those involved in microbe recognition and host defense are upregulated. In addition, aging is associated with an overall increase in expression of microglial genes involved in neuroprotection.
Published Version: doi:10.1038/nn.3554
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