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dc.contributor.authorPeng, Lishengen_US
dc.contributor.authorLiu, Huishengen_US
dc.contributor.authorRuan, Hongyuen_US
dc.contributor.authorTepp, William H.en_US
dc.contributor.authorStoothoff, William H.en_US
dc.contributor.authorBrown, Robert H.en_US
dc.contributor.authorJohnson, Eric A.en_US
dc.contributor.authorDong, Minen_US
dc.contributor.authorYao, Wei-Dongen_US
dc.contributor.authorZhang, Su-Chunen_US
dc.date.accessioned2014-07-07T17:03:20Z
dc.date.issued2014en_US
dc.identifier.citationPeng, Lisheng, Huisheng Liu, Hongyu Ruan, William H. Tepp, William H. Stoothoff, Robert H. Brown, Eric A. Johnson, Wei-Dong Yao, Su-Chun Zhang, and Min Dong. 2014. “Cytotoxicity of Botulinum Neurotoxins Reveals a Direct Role of Syntaxin 1 and SNAP-25 in Neuron Survival.” Nature communications 4 (1): 1472. doi:10.1038/ncomms2462. http://dx.doi.org/10.1038/ncomms2462.en
dc.identifier.issn2041-1723en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12406680
dc.description.abstractBotulinum neurotoxins (BoNT/A-G) are well-known to act by blocking synaptic vesicle exocytosis. Whether BoNTs disrupt additional neuronal functions has not been addressed. Here we report that cleavage of syntaxin 1 (Syx 1) by BoNT/C and cleavage of SNAP-25 by BoNT/E both induce degeneration of cultured rodent and human neurons. Furthermore, although SNAP-25 cleaved by BoNT/A can still support neuron survival, it has reduced capacity to tolerate additional mutations and also fails to pair with syntaxin isoforms other than Syx 1. Syx 1 and SNAP-25 are well-known for mediating synaptic vesicle exocytosis, but we found that neuronal death is due to blockage of plasma membrane recycling processes that share Syx 1/SNAP-25 for exocytosis, independent of blockage of synaptic vesicle exocytosis. These findings reveal neuronal cytotoxicity for a subset of BoNTs and directly link Syx 1/SNAP-25 to neuron survival as the prevalent SNARE proteins mediating multiple fusion events on neuronal plasma membranes.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/ncomms2462en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052923/pdf/en
dash.licenseLAAen_US
dc.titleCytotoxicity of Botulinum Neurotoxins Reveals a Direct Role of Syntaxin 1 and SNAP-25 in Neuron Survivalen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature communicationsen
dash.depositing.authorPeng, Lishengen_US
dc.date.available2014-07-07T17:03:20Z
dc.identifier.doi10.1038/ncomms2462*
dash.contributor.affiliatedYao, Wei-Dong
dash.contributor.affiliatedRuan, Hongyu
dash.contributor.affiliatedPeng, Lisheng
dash.contributor.affiliatedDong, Min


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