dc.contributor.author | Peng, Lisheng | en_US |
dc.contributor.author | Liu, Huisheng | en_US |
dc.contributor.author | Ruan, Hongyu | en_US |
dc.contributor.author | Tepp, William H. | en_US |
dc.contributor.author | Stoothoff, William H. | en_US |
dc.contributor.author | Brown, Robert H. | en_US |
dc.contributor.author | Johnson, Eric A. | en_US |
dc.contributor.author | Dong, Min | en_US |
dc.contributor.author | Yao, Wei-Dong | en_US |
dc.contributor.author | Zhang, Su-Chun | en_US |
dc.date.accessioned | 2014-07-07T17:03:20Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Peng, Lisheng, Huisheng Liu, Hongyu Ruan, William H. Tepp, William H. Stoothoff, Robert H. Brown, Eric A. Johnson, Wei-Dong Yao, Su-Chun Zhang, and Min Dong. 2014. “Cytotoxicity of Botulinum Neurotoxins Reveals a Direct Role of Syntaxin 1 and SNAP-25 in Neuron Survival.” Nature communications 4 (1): 1472. doi:10.1038/ncomms2462. http://dx.doi.org/10.1038/ncomms2462. | en |
dc.identifier.issn | 2041-1723 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12406680 | |
dc.description.abstract | Botulinum neurotoxins (BoNT/A-G) are well-known to act by blocking synaptic vesicle exocytosis. Whether BoNTs disrupt additional neuronal functions has not been addressed. Here we report that cleavage of syntaxin 1 (Syx 1) by BoNT/C and cleavage of SNAP-25 by BoNT/E both induce degeneration of cultured rodent and human neurons. Furthermore, although SNAP-25 cleaved by BoNT/A can still support neuron survival, it has reduced capacity to tolerate additional mutations and also fails to pair with syntaxin isoforms other than Syx 1. Syx 1 and SNAP-25 are well-known for mediating synaptic vesicle exocytosis, but we found that neuronal death is due to blockage of plasma membrane recycling processes that share Syx 1/SNAP-25 for exocytosis, independent of blockage of synaptic vesicle exocytosis. These findings reveal neuronal cytotoxicity for a subset of BoNTs and directly link Syx 1/SNAP-25 to neuron survival as the prevalent SNARE proteins mediating multiple fusion events on neuronal plasma membranes. | en |
dc.language.iso | en_US | en |
dc.relation.isversionof | doi:10.1038/ncomms2462 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052923/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Cytotoxicity of Botulinum Neurotoxins Reveals a Direct Role of Syntaxin 1 and SNAP-25 in Neuron Survival | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Nature communications | en |
dash.depositing.author | Peng, Lisheng | en_US |
dc.date.available | 2014-07-07T17:03:20Z | |
dc.identifier.doi | 10.1038/ncomms2462 | * |
dash.contributor.affiliated | Yao, Wei-Dong | |
dash.contributor.affiliated | Ruan, Hongyu | |
dash.contributor.affiliated | Peng, Lisheng | |
dash.contributor.affiliated | Dong, Min | |