Measuring cell-type specific differential methylation in human brain tissue
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Author
Montaño, Carolina M
Irizarry, Rafael A
Talbot, Konrad
Gur, Raquel E
Feinberg, Andrew P
Taub, Margaret A
Published Version
https://doi.org/10.1186/gb-2013-14-8-r94Metadata
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Montaño, Carolina M, Rafael A Irizarry, Walter E Kaufmann, Konrad Talbot, Raquel E Gur, Andrew P Feinberg, and Margaret A Taub. 2013. “Measuring cell-type specific differential methylation in human brain tissue.” Genome Biology 14 (8): R94. doi:10.1186/gb-2013-14-8-r94. http://dx.doi.org/10.1186/gb-2013-14-8-r94.Abstract
The behavior of epigenetic mechanisms in the brain is obscured by tissue heterogeneity and disease-related histological changes. Not accounting for these confounders leads to biased results. We develop a statistical methodology that estimates and adjusts for celltype composition by decomposing neuronal and non-neuronal differential signal. This method provides a conceptual framework for deconvolving heterogeneous epigenetic data from postmortem brain studies. We apply it to find cell-specific differentially methylated regions between prefrontal cortex and hippocampus. We demonstrate the utility of the method on both Infinium 450k and CHARM data.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054676/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:12406694
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