Role of Tet1 in genomic imprinting erasure

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Role of Tet1 in genomic imprinting erasure

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Title: Role of Tet1 in genomic imprinting erasure
Author: Yamaguchi, Shinpei; Shen, Li; Liu, Yuting; Sendler, Damian; Zhang, Yi

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Citation: Yamaguchi, Shinpei, Li Shen, Yuting Liu, Damian Sendler, and Yi Zhang. 2013. “Role of Tet1 in genomic imprinting erasure.” Nature 504 (7480): 460-464. doi:10.1038/nature12805. http://dx.doi.org/10.1038/nature12805.
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Abstract: Genomic imprinting is an allele-specific gene expression system important for mammalian development and function 1. The molecular basis of genomic imprinting is allele-specific DNA methylation 1,2. While it is well known that the de novo DNA methyltransferases Dnmt3a/b are responsible for the establishment of genomic imprinting 3, how the methylation mark is erased during primordial germ cell (PGC) reprogramming remains a mystery. Tet1 is one of the ten-eleven translocation family proteins, which have the capacity to oxidize 5-methylcytosine (5mC) 4-6, specifically expressed in reprogramming PGCs 7. Here we report that Tet1 plays a critical role in the erasure of genomic imprinting. We show that despite their identical genotype, progenies derived from mating between Tet1-KO males and wild-type females exhibit a number of variable phenotypes including placental, fetal and postnatal growth defects, and early embryonic lethality. These defects are, at least in part, caused by the dysregulation of imprinted genes, such as Peg10 and Peg3, which exhibit aberrant hypermethylation in the paternal allele of differential methylated regions (DMRs). RNA-seq reveals extensive dysregulation of imprinted genes in the next generation due to paternal loss function of Tet1. Genome-wide DNA methylation analysis of E13.5 PGCs and sperms of Tet1-KO mice revealed hypermethylation of DMRs of imprinted genes in sperm, which can be traced back to PGCs. Analysis of the DNA methylation dynamics in reprogramming PGCs suggests that Tet1 functions to wipe out remaining methylation, including imprinted genes, at the late reprogramming stage. We further provide evidence supporting Tet1's role in the erasure of paternal imprints in female germline. Thus, our study establishes a critical function of Tet1 in genomic imprinting erasure.
Published Version: doi:10.1038/nature12805
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957231/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12406729
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