In Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Induction

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Author
Ohba, Kenji
Ichiyama, Koji
Yajima, Misako
Gemma, Nobuhiro
Nikaido, Masaru
Wu, Qingqing
Chong, PeiPei
Mori, Seiichiro
Yamamoto, Rain
Wong, John Eu Li
Yamamoto, Naoki
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1371/journal.pone.0097787Metadata
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Ohba, K., K. Ichiyama, M. Yajima, N. Gemma, M. Nikaido, Q. Wu, P. Chong, et al. 2014. “In Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Induction.” PLoS ONE 9 (5): e97787. doi:10.1371/journal.pone.0097787. http://dx.doi.org/10.1371/journal.pone.0097787.Abstract
High prevalence of infection with high-risk human papilloma virus (HPV) ranging from 25 to 100% (average 31%) was observed in breast cancer (BC) patients in Singapore using novel DNA chip technology. Early stage of BC demonstrated higher HPV positivity, and BC positive for estrogen receptor (ER) showed significantly higher HPV infection rate. This unique association of HPV with BC in vivo prompted us to investigate a possible involvement of HPV in early stages of breast carcinogenesis. Using normal breast epithelial cells stably transfected with HPV-18, we showed apparent upregulation of mRNA for the cytidine deaminase, APOBEC3B (A3B) which is reported to be a source of mutations in BC. HPV-induced A3B overexpression caused significant γH2AX focus formation, and DNA breaks which were cancelled by shRNA to HPV18 E6, E7 and A3B. These results strongly suggest an active involvement of HPV in the early stage of BC carcinogenesis via A3B induction.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032256/pdf/Terms of Use
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