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dc.contributor.authorOhba, Kenjien_US
dc.contributor.authorIchiyama, Kojien_US
dc.contributor.authorYajima, Misakoen_US
dc.contributor.authorGemma, Nobuhiroen_US
dc.contributor.authorNikaido, Masaruen_US
dc.contributor.authorWu, Qingqingen_US
dc.contributor.authorChong, PeiPeien_US
dc.contributor.authorMori, Seiichiroen_US
dc.contributor.authorYamamoto, Rainen_US
dc.contributor.authorWong, John Eu Lien_US
dc.contributor.authorYamamoto, Naokien_US
dc.date.accessioned2014-07-07T18:13:26Z
dc.date.issued2014en_US
dc.identifier.citationOhba, K., K. Ichiyama, M. Yajima, N. Gemma, M. Nikaido, Q. Wu, P. Chong, et al. 2014. “In Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Induction.” PLoS ONE 9 (5): e97787. doi:10.1371/journal.pone.0097787. http://dx.doi.org/10.1371/journal.pone.0097787.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12406885
dc.description.abstractHigh prevalence of infection with high-risk human papilloma virus (HPV) ranging from 25 to 100% (average 31%) was observed in breast cancer (BC) patients in Singapore using novel DNA chip technology. Early stage of BC demonstrated higher HPV positivity, and BC positive for estrogen receptor (ER) showed significantly higher HPV infection rate. This unique association of HPV with BC in vivo prompted us to investigate a possible involvement of HPV in early stages of breast carcinogenesis. Using normal breast epithelial cells stably transfected with HPV-18, we showed apparent upregulation of mRNA for the cytidine deaminase, APOBEC3B (A3B) which is reported to be a source of mutations in BC. HPV-induced A3B overexpression caused significant γH2AX focus formation, and DNA breaks which were cancelled by shRNA to HPV18 E6, E7 and A3B. These results strongly suggest an active involvement of HPV in the early stage of BC carcinogenesis via A3B induction.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0097787en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032256/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and life sciencesen
dc.subjectBiochemistryen
dc.subjectDNAen
dc.subjectDNA damageen
dc.subjectCell Biologyen
dc.subjectMolecular Cell Biologyen
dc.subjectGeneticsen
dc.subjectImmunologyen
dc.subjectImmunity to Infectionsen
dc.subjectMicrobiologyen
dc.subjectVirologyen
dc.subjectVirus Effects on Host Gene Expressionen
dc.subjectViruses and Canceren
dc.subjectMolecular Biologyen
dc.subjectMedicine and Health Sciencesen
dc.subjectInfectious Diseasesen
dc.subjectSexually Transmitted Diseasesen
dc.subjectHuman Papillomavirus Infectionen
dc.subjectViral Diseasesen
dc.titleIn Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Inductionen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dc.date.available2014-07-07T18:13:26Z
dc.identifier.doi10.1371/journal.pone.0097787*
dash.authorsorderedfalse


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