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dc.contributor.authorLI, FEIen_US
dc.contributor.authorXIA, KEen_US
dc.contributor.authorSHEIKH, MD SAYED ALIen_US
dc.contributor.authorCHENG, JINFANGen_US
dc.contributor.authorLI, CHUANCHANGen_US
dc.contributor.authorYANG, TIANLUNen_US
dc.date.accessioned2014-07-07T18:14:25Z
dc.date.issued2014en_US
dc.identifier.citationLI, FEI, KE XIA, MD SAYED ALI SHEIKH, JINFANG CHENG, CHUANCHANG LI, and TIANLUN YANG. 2014. “Retinol binding protein 4 promotes hyperinsulinism-induced proliferation of rat aortic smooth muscle cells.” Molecular Medicine Reports 9 (5): 1634-1640. doi:10.3892/mmr.2014.2028. http://dx.doi.org/10.3892/mmr.2014.2028.en
dc.identifier.issn1791-2997en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12406945
dc.description.abstractRecent studies have suggested that retinol binding protein 4 (RBP4), an adipocytokine related to insulin resistance (IR), may play an important role in the development of atherosclerosis and cardiovascular diseases (CVD). Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is one of the most common causes of atherosclerosis. Hyperinsulinism promotes proliferation of VSMCs through the MAPK pathway. However, whether RBP4 is involved in insulin-induced proliferation of VSMCs leading to atherosclerosis remains unclear. In the present study, we evaluated the role of RBP4 and the potential relevance of signaling pathways in this process. Different concentrations of RBP4 (1 and 4 μg/ml) were added to rat aortic smooth muscle cells (RASMCs) during insulin-induced proliferation. The levels of cell growth signaling pathway proteins ERK1/2, p-ERK1/2, JAK2, p-JAK2, STAT3 and p-STAT3 were assessed by western blotting in order to identify the pathway(s) that are activated during insulin-induced proliferation. The specific inhibitors of ERK1/2 (PD98059) and JAK2 (AG490) were used to confirm our findings. Insulin induced proliferation of RASMCs in a concentration- and time-dependent manner, and increased the expression of ERK1/2, p-ERK1/2, JAK2, p-JAK2, STAT3 and p-STAT3 in a time-dependent manner. RBP4 enhanced insulin-induced proliferation of RASMCs and expression of p-ERK1/2 and p-JAK2. RBP4-induced proliferation of RASMCs was reduced by the ERK1/2 inhibitor, while it was unaffected by the JAK2 inhibitor. These results suggest that RBP4 mediates VSMC proliferation induced by insulin via activation of the MAPK pathway, and highlight RBP4 as a modulator of atherosclerosis in hyperinsulinemia, therby enhancing our understanding on a number of unexpected aspects of CVD.en
dc.language.isoen_USen
dc.publisherD.A. Spandidosen
dc.relation.isversionofdoi:10.3892/mmr.2014.2028en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020478/pdf/en
dash.licenseLAAen_US
dc.subjectretinol binding protein 4en
dc.subjecthyperinsulinismen
dc.subjectvascular smooth muscle cellsen
dc.subjectproliferationen
dc.subjectsignaling pathwayen
dc.titleRetinol binding protein 4 promotes hyperinsulinism-induced proliferation of rat aortic smooth muscle cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalMolecular Medicine Reportsen
dash.depositing.authorXIA, KEen_US
dc.date.available2014-07-07T18:14:25Z
dc.identifier.doi10.3892/mmr.2014.2028*
dash.authorsorderedfalse
dash.contributor.affiliatedXIA, KE


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