Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1

DSpace/Manakin Repository

Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1

Citable link to this page

 

 
Title: Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1
Author: Truong, Tan N.; Li, Hannah; Hong, Young-Kwon; Chen, Lu

Note: Order does not necessarily reflect citation order of authors.

Citation: Truong, Tan N., Hannah Li, Young-Kwon Hong, and Lu Chen. 2014. “Novel Characterization and Live Imaging of Schlemm's Canal Expressing Prox-1.” PLoS ONE 9 (5): e98245. doi:10.1371/journal.pone.0098245. http://dx.doi.org/10.1371/journal.pone.0098245.
Full Text & Related Files:
Abstract: Schlemm's canal is an important structure of the conventional aqueous humor outflow pathway and is critically involved in regulating the intraocular pressure. In this study, we report a novel finding that prospero homeobox protein 1 (Prox-1), the master control gene for lymphatic development, is expressed in Schlemm's canal. Moreover, we provide a novel in vivo method of visualizing Schlemm's canal using a transgenic mouse model of Prox-1-green fluorescent protein (GFP). The anatomical location of Prox-1+ Schlemm's canal was further confirmed by in vivo gonioscopic examination and ex vivo immunohistochemical analysis. Additionally, we show that the Schlemm's canal is distinguishable from typical lymphatic vessels by lack of lymphatic vessel endothelial hyaluronan receptor (LYVE-1) expression and absence of apparent sprouting reaction when inflammatory lymphangiogenesis occurred in the cornea. Taken together, our findings offer new insights into Schlemm's canal and provide a new experimental model for live imaging of this critical structure to help further our understanding of the aqueous humor outflow. This may lead to new avenues toward the development of novel therapeutic intervention for relevant diseases, most notably glaucoma.
Published Version: doi:10.1371/journal.pone.0098245
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4020937/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12406947
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters