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dc.contributor.authorZhu, Binen_US
dc.date.accessioned2014-07-07T18:14:56Z
dc.date.issued2014en_US
dc.identifier.citationZhu, Bin. 2014. “Bacteriophage T7 DNA polymerase – sequenase.” Frontiers in Microbiology 5 (1): 181. doi:10.3389/fmicb.2014.00181. http://dx.doi.org/10.3389/fmicb.2014.00181.en
dc.identifier.issn1664-302Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12407012
dc.description.abstractAn ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase encoded by bacteriophage T7 is naturally endowed with or can be engineered to have all these characteristics. The chemically or genetically modified enzyme (Sequenase) expedited significantly the development of DNA sequencing technology. This article reviews the history of studies on T7 DNA polymerase with emphasis on the serial key steps leading to its use in DNA sequencing. Lessons from the study and development of T7 DNA polymerase have and will continue to enlighten the characterization of novel DNA polymerases from newly discovered microbes and their modification for use in biotechnology.en
dc.language.isoen_USen
dc.publisherFrontiers Media S.A.en
dc.relation.isversionofdoi:10.3389/fmicb.2014.00181en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997047/pdf/en
dash.licenseLAAen_US
dc.subjectReview Articleen
dc.subjectbacteriophage T7en
dc.subjectDNA polymeraseen
dc.subjectsequenaseen
dc.subjectDNA sequencingen
dc.subjectmarine phagesen
dc.titleBacteriophage T7 DNA polymerase – sequenaseen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalFrontiers in Microbiologyen
dash.depositing.authorZhu, Binen_US
dc.date.available2014-07-07T18:14:56Z
dc.identifier.doi10.3389/fmicb.2014.00181*
dash.contributor.affiliatedZhu, Bin


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