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dc.contributor.authorTritsch, Nicolas Xen_US
dc.contributor.authorOh, Won-Jongen_US
dc.contributor.authorGu, Chenghuaen_US
dc.contributor.authorSabatini, Bernardo Len_US
dc.date.accessioned2014-07-07T18:15:00Z
dc.date.issued2014en_US
dc.identifier.citationTritsch, Nicolas X, Won-Jong Oh, Chenghua Gu, and Bernardo L Sabatini. 2014. “Midbrain dopamine neurons sustain inhibitory transmission using plasma membrane uptake of GABA, not synthesis.” eLife 3 (1): e01936. doi:10.7554/eLife.01936. http://dx.doi.org/10.7554/eLife.01936.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12407019
dc.description.abstractSynaptic transmission between midbrain dopamine neurons and target neurons in the striatum is essential for the selection and reinforcement of movements. Recent evidence indicates that nigrostriatal dopamine neurons inhibit striatal projection neurons by releasing a neurotransmitter that activates GABAA receptors. Here, we demonstrate that this phenomenon extends to mesolimbic afferents, and confirm that the released neurotransmitter is GABA. However, the GABA synthetic enzymes GAD65 and GAD67 are not detected in midbrain dopamine neurons. Instead, these cells express the membrane GABA transporters mGAT1 (Slc6a1) and mGAT4 (Slc6a11) and inhibition of these transporters prevents GABA co-release. These findings therefore indicate that GABA co-release is a general feature of midbrain dopaminergic neurons that relies on GABA uptake from the extracellular milieu as opposed to de novo synthesis. This atypical mechanism may confer dopaminergic neurons the flexibility to differentially control GABAergic transmission in a target-dependent manner across their extensive axonal arbors. DOI: http://dx.doi.org/10.7554/eLife.01936.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.01936en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001323/pdf/en
dash.licenseLAAen_US
dc.subjectbasal gangliaen
dc.subjectdopamineen
dc.subjectGABAen
dc.subjectstriatumen
dc.subjectco-releaseen
dc.subjectGATen
dc.subjectmouseen
dc.titleMidbrain dopamine neurons sustain inhibitory transmission using plasma membrane uptake of GABA, not synthesisen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorTritsch, Nicolas Xen_US
dc.date.available2014-07-07T18:15:00Z
dc.identifier.doi10.7554/eLife.01936*
dash.contributor.affiliatedTritsch, Nicolas X
dash.contributor.affiliatedSabatini, Bernardo
dash.contributor.affiliatedGu, Chenghua


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