Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation

DSpace/Manakin Repository

Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation

Citable link to this page

 

 
Title: Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation
Author: Tan, Shen Mynn; Altschuler, Gabriel; Zhao, Tian Yun; Ang, Haw Siang; Yang, Henry; Lim, Bing; Vardy, Leah; Hide, Winston; Thomson, Andrew M.; Lareu, Ricky R.

Note: Order does not necessarily reflect citation order of authors.

Citation: Tan, Shen Mynn, Gabriel Altschuler, Tian Yun Zhao, Haw Siang Ang, Henry Yang, Bing Lim, Leah Vardy, Winston Hide, Andrew M. Thomson, and Ricky R. Lareu. 2014. “Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.” Nucleic Acids Research 42 (12): 7997-8007. doi:10.1093/nar/gku430. http://dx.doi.org/10.1093/nar/gku430.
Full Text & Related Files:
Abstract: LIN28 function is fundamental to the activity and behavior of human embryonic stem cells (hESCs) and induced pluripotent stem cells. Its main roles in these cell types are the regulation of translational efficiency and let-7 miRNA maturation. However, LIN28-associated mRNA cargo shifting and resultant regulation of translational efficiency upon the initiation of differentiation remain unknown. An RNA-immunoprecipitation and microarray analysis protocol, eRIP, that has high specificity and sensitivity was developed to test endogenous LIN28-associated mRNA cargo shifting. A combined eRIP and polysome analysis of early stage differentiation of hESCs with two distinct differentiation cues revealed close similarities between the dynamics of LIN28 association and translational modulation of genes involved in the Wnt signaling, cell cycle, RNA metabolism and proteasomal pathways. Our data demonstrate that change in translational efficiency is a major contributor to early stages of differentiation of hESCs, in which LIN28 plays a central role. This implies that eRIP analysis of LIN28-associated RNA cargoes may be used for rapid functional quality control of pluripotent stem cells under manufacture for therapeutic applications.
Published Version: doi:10.1093/nar/gku430
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081066/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12717412
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters