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dc.contributor.authorVinayagam, Arunachalamen_US
dc.contributor.authorZirin, Jonathanen_US
dc.contributor.authorRoesel, Charlesen_US
dc.contributor.authorHu, Yanhuien_US
dc.contributor.authorYilmazel, Baharen_US
dc.contributor.authorSamsonova, Anastasia A.en_US
dc.contributor.authorNeumüller, Ralph A.en_US
dc.contributor.authorMohr, Stephanie E.en_US
dc.contributor.authorPerrimon, Norberten_US
dc.date.accessioned2014-08-13T13:59:15Z
dc.date.issued2013en_US
dc.identifier.citationVinayagam, Arunachalam, Jonathan Zirin, Charles Roesel, Yanhui Hu, Bahar Yilmazel, Anastasia A. Samsonova, Ralph A. Neumüller, Stephanie E. Mohr, and Norbert Perrimon. 2013. “Integrating protein-protein interaction networks with phenotypes reveals signs of interactions.” Nature methods 11 (1): 10.1038/nmeth.2733. doi:10.1038/nmeth.2733. http://dx.doi.org/10.1038/nmeth.2733.en
dc.identifier.issn1548-7091en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12717461
dc.description.abstractA major objective of systems biology is to organize molecular interactions as networks and to characterize information-flow within networks. We describe a computational framework to integrate protein-protein interaction (PPI) networks and genetic screens to predict the “signs” of interactions (i.e. activation/inhibition relationships). We constructed a Drosophila melanogaster signed PPI network, consisting of 6,125 signed PPIs connecting 3,352 proteins that can be used to identify positive and negative regulators of signaling pathways and protein complexes. We identified an unexpected role for the metabolic enzymes Enolase and Aldo-keto reductase as positive and negative regulators of proteolysis, respectively. Characterization of the activation/inhibition relationships between physically interacting proteins within signaling pathways will impact our understanding of many biological functions, including signal transduction and mechanisms of disease.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nmeth.2733en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877743/pdf/en
dash.licenseLAAen_US
dc.titleIntegrating protein-protein interaction networks with phenotypes reveals signs of interactionsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature methodsen
dash.depositing.authorZirin, Jonathanen_US
dc.date.available2014-08-13T13:59:15Z
dc.identifier.doi10.1038/nmeth.2733*
dash.contributor.affiliatedZirin, Jonathan
dash.contributor.affiliatedMohr, Stephanie
dash.contributor.affiliatedHu, Yanhui
dash.contributor.affiliatedPerrimon, Norbert


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