dc.contributor.author | Bodor, Dani L | en_US |
dc.contributor.author | Mata, João F | en_US |
dc.contributor.author | Sergeev, Mikhail | en_US |
dc.contributor.author | David, Ana Filipa | en_US |
dc.contributor.author | Salimian, Kevan J | en_US |
dc.contributor.author | Panchenko, Tanya | en_US |
dc.contributor.author | Cleveland, Don W | en_US |
dc.contributor.author | Black, Ben E | en_US |
dc.contributor.author | Shah, Jagesh V | en_US |
dc.contributor.author | Jansen, Lars ET | en_US |
dc.date.accessioned | 2014-08-13T13:59:35Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Bodor, Dani L, João F Mata, Mikhail Sergeev, Ana Filipa David, Kevan J Salimian, Tanya Panchenko, Don W Cleveland, Ben E Black, Jagesh V Shah, and Lars ET Jansen. 2014. “The quantitative architecture of centromeric chromatin.” eLife 3 (1): e02137. doi:10.7554/eLife.02137. http://dx.doi.org/10.7554/eLife.02137. | en |
dc.identifier.issn | 2050-084X | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12717501 | |
dc.description.abstract | The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation. DOI: http://dx.doi.org/10.7554/eLife.02137.001 | en |
dc.language.iso | en_US | en |
dc.publisher | eLife Sciences Publications, Ltd | en |
dc.relation.isversionof | doi:10.7554/eLife.02137 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091408/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | centromere | en |
dc.subject | CENP-A | en |
dc.subject | epigenetics | en |
dc.subject | molecular counting | en |
dc.subject | quantitative microscopy | en |
dc.subject | histone variant | en |
dc.subject | human | en |
dc.title | The quantitative architecture of centromeric chromatin | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | eLife | en |
dash.depositing.author | Sergeev, Mikhail | en_US |
dc.date.available | 2014-08-13T13:59:35Z | |
dc.identifier.doi | 10.7554/eLife.02137 | * |
dash.contributor.affiliated | Sergeev, Mikhail | |
dash.contributor.affiliated | Shah, Jagesh | |