NADPH oxidases regulate cell growth and migration in myeloid cells transformed by oncogenic tyrosine kinases
Reddy, Mamatha M.
Fernandes, Margret S.
Levine, Ross L.
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CitationReddy, Mamatha M., Margret S. Fernandes, Ravi Salgia, Ross L. Levine, James D. Griffin, and Martin Sattler. 2014. “NADPH oxidases regulate cell growth and migration in myeloid cells transformed by oncogenic tyrosine kinases.” Leukemia 25 (2): 281-289. doi:10.1038/leu.2010.263. http://dx.doi.org/10.1038/leu.2010.263.
AbstractTransformation by tyrosine kinase oncogenes in myeloid malignancies, including BCR-ABL in chronic myeloid leukemia, FLT3ITD in acute myeloid leukemia (AML) or JAK2V617F in myeloproliferative neoplasms (MPN), is associated with increased growth and cytoskeletal abnormalities. Using targeted approaches against components of the superoxide-producing NADPH-oxidases, including NOX2, NOX4 and the common p22phox subunit of NOX1-4, myeloid cells were found to display reduced cell growth and spontaneous migration. Consistent with a role of NOX as regulators of membrane proximal signaling events in non-phagocytic cells, NOX2 and NOX4 were not involved in the excess production of intracellular reactive oxygen species and did not significantly increase oxygen consumption. All NOX family members are controlled in part through levels of the rate-limiting substrate NADPH, which was found to be significantly elevated in tyrosine kinase oncogene transformed cells. Also, reduced phosphorylation of the actin filament crosslinking protein MARCKS in response to suppression of p22phox hints at a novel effector of NOX signaling. MARCKS was also found to be required for increased migration. Overall, these data suggest a model whereby NOX links metabolic NADPH production to cellular events that directly contribute to transformation.
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