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dc.contributor.authorAmento, Edward P.
dc.contributor.authorEhsani, Niloofar
dc.contributor.authorPalmer, Helen
dc.contributor.authorLibby, Peter
dc.date.accessioned2014-08-29T14:50:12Z
dash.embargo.terms10000-01-01
dc.date.issued1991
dc.identifier.citationAmento, Edward P., Niloofar Ehsani, Helen Palmer, and Peter Libby. 1991. “Cytokines and Growth Factors Positively and Negatively Regulate Interstitial Collagen Gene Expression in Human Vascular Smooth Muscle Cells.” Arteriosclerosis, Thrombosis, and Vascular Biology 11 (5) (September 1): 1223–1230. doi:10.1161/01.atv.11.5.1223. http://dx.doi.org/10.1161/01.ATV.11.5.1223.en_US
dc.identifier.issn1079-5642en_US
dc.identifier.issn1524-4636en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12760224
dc.description.abstractHuman atheromas accumulate extracellular matrix proteins such as collagen types I and III. We tested whether cytokines or growth factors produced by cells found in human atherosclerotic plaques alter collagen gene expression in vascular smooth muscle cells (VSMCs), which produce the blood vessel matrix. Interleukin-1 (IL-1, 1-10 ng/ml) modestly increased the synthesis of collagens I and III (measured by tritiated proline incorporation into specific electrophoretic bands), whereas transforming growth factor-\(\beta\) (TGF-\(\beta\)) or platelet-derived growth factor (PDGF) markedly stimulated production of these interstitial collagens. Interferon \(\gamma\) (IFN-\(\gamma\)), a product of activated T cells found in atheromas, selectively alters several VSMC functions. For example, this cytokine reduces growth of VSMCs, decreases alpha-actin gene expression, and induces VSMC expression of class II histocompatibility antigens. We report here that IFN-\(\gamma\) also inhibits basal as well as IL-1-, PDGF-, or TGF-\(\beta\)-stimulated collagen I and III synthesis by human VSMCs. TGF-\(\beta\), the most potent stimulator of collagen synthesis studied here, raised the level of collagen III mRNA in VSMCs 4.8-fold (determined by densitometry of Northern blots), whereas exposure to both TGF-\(\beta\) and IFN-\(\gamma\) reduced this mRNA to 0.5 of basal level. Locally produced cytokines and growth factors may thus modify matrix accumulation during atherogenesis by stimulating or suppressing expression of interstitial collagen mRNA and protein by VSMCs.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Heart Associationen_US
dc.relation.isversionofdoi:10.1161/01.ATV.11.5.1223en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/1911708en_US
dash.licenseMETA_ONLY
dc.titleCytokines and Growth Factors Positively and Negatively Regulate Interstitial Collagen Gene Expression in Human Vascular Smooth Muscle Cellsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalArteriosclerosis, Thrombosis, and Vascular Biologyen_US
dash.depositing.authorLibby, Peter
dash.embargo.until10000-01-01
dc.date.available2014-08-29T14:50:12Z
dc.identifier.doi10.1161/01.ATV.11.5.1223*
dash.contributor.affiliatedLibby, Peter
dc.identifier.orcid0000-0002-1502-502X


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