Topological Organization of Multi-chromosomal Regions by Firre

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Author
Hacisuleyman, Ezgi
Trapnell, Cole
Williams, Adam
Henao-Mejia, Jorge
Sun, Lei
McClanahan, Patrick
Morse, Michael
Engreitz, Jesse
Guttman, Mitch
Lodish, Harvey F.
Flavell, Richard
Raj, Arjun
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/nsmb.2764Metadata
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Hacisuleyman, E., L. A. Goff, C. Trapnell, A. Williams, J. Henao-Mejia, L. Sun, P. McClanahan, et al. 2014. “Topological Organization of Multi-chromosomal Regions by Firre.” Nature structural & molecular biology 21 (2): 198-206. doi:10.1038/nsmb.2764. http://dx.doi.org/10.1038/nsmb.2764.Abstract
RNA is known to be an abundant and important structural component of the nuclear matrix, including long noncoding RNAs (lncRNA). Yet the molecular identities, functional roles, and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear matrix factor hnRNPU, through a 156 bp repeating sequence and Firre localizes across a ~5 Mb domain on the X-chromosome. We further observed Firre localization across at least five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X-chromosome. Both genetic deletion of the Firre locus or knockdown of hnRNPU resulted in loss of co-localization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950333/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785789
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