The Scavenger Receptor MARCO Modulates TLR-Induced Responses in Dendritic Cells

DSpace/Manakin Repository

The Scavenger Receptor MARCO Modulates TLR-Induced Responses in Dendritic Cells

Citable link to this page

 

 
Title: The Scavenger Receptor MARCO Modulates TLR-Induced Responses in Dendritic Cells
Author: Kissick, Haydn T.; Dunn, Laura K.; Ghosh, Sanjukta; Nechama, Morris; Kobzik, Lester; Arredouani, Mohamed S.

Note: Order does not necessarily reflect citation order of authors.

Citation: Kissick, Haydn T., Laura K. Dunn, Sanjukta Ghosh, Morris Nechama, Lester Kobzik, and Mohamed S. Arredouani. 2014. “The Scavenger Receptor MARCO Modulates TLR-Induced Responses in Dendritic Cells.” PLoS ONE 9 (8): e104148. doi:10.1371/journal.pone.0104148. http://dx.doi.org/10.1371/journal.pone.0104148.
Full Text & Related Files:
Abstract: The scavenger receptor MARCO mediates macrophage recognition and clearance of pathogens and their polyanionic ligands. However, recent studies demonstrate MARCO expression and function in dendritic cells, suggesting MARCO might serve to bridge innate and adaptive immunity. To gain additional insight into the role of MARCO in dendritic cell activation and function, we profiled transcriptomes of mouse splenic dendritic cells obtained from MARCO deficient mice and their wild type counterparts under resting and activating conditions. In silico analysis uncovered major alterations in gene expression in MARCO deficient dendritic cells resulting in dramatic alterations in key dendritic cell-specific pathways and functions. Specifically, changes in CD209, FCGR4 and Complement factors can have major consequences on DC-mediated innate responses. Notably, these perturbations were magnified following activation with the TLR-4 agonist lipopolysaccharide. To validate our in silico data, we challenged DC‘s with various agonists that recognize all mouse TLRs and assessed expression of a set of immune and inflammatory marker genes. This approach identified a differential contribution of MARCO to TLR activation and validated a major role for MARCO in mounting an inflammatory response. Together, our data demonstrate that MARCO differentially affects TLR-induced DC activation and suggest targeting of MARCO could lead to different outcomes that depend on the inflammatory context encountered by DC.
Published Version: doi:10.1371/journal.pone.0104148
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121322/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785818
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters