Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space

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Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space

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Title: Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space
Author: Liu, Zhen; Xing, Dong; Su, Qian Peter; Zhu, Yun; Zhang, Jiamei; Kong, Xinyu; Xue, Boxin; Wang, Sheng; Sun, Hao; Tao, Yile; Sun, Yujie

Note: Order does not necessarily reflect citation order of authors.

Citation: Liu, Z., D. Xing, Q. P. Su, Y. Zhu, J. Zhang, X. Kong, B. Xue, et al. 2014. “Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space.” Nature Communications 5 (1): 4443. doi:10.1038/ncomms5443. http://dx.doi.org/10.1038/ncomms5443.
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Abstract: Imaging the location and dynamics of individual interacting protein pairs is essential but often difficult because of the fluorescent background from other paired and non-paired molecules, particularly in the sub-diffraction cellular space. Here we develop a new method combining bimolecular fluorescence complementation and photoactivated localization microscopy for super-resolution imaging and single-molecule tracking of specific protein–protein interactions. The method is used to study the interaction of two abundant proteins, MreB and EF-Tu, in Escherichia coli cells. The super-resolution imaging shows interesting distribution and domain sizes of interacting MreB–EF-Tu pairs as a subpopulation of total EF-Tu. The single-molecule tracking of MreB, EF-Tu and MreB–EF-Tu pairs reveals intriguing localization-dependent heterogonous dynamics and provides valuable insights to understanding the roles of MreB–EF-Tu interactions.
Published Version: doi:10.1038/ncomms5443
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109008/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785855
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