dc.contributor.author | Liu, Zhen | en_US |
dc.contributor.author | Xing, Dong | en_US |
dc.contributor.author | Su, Qian Peter | en_US |
dc.contributor.author | Zhu, Yun | en_US |
dc.contributor.author | Zhang, Jiamei | en_US |
dc.contributor.author | Kong, Xinyu | en_US |
dc.contributor.author | Xue, Boxin | en_US |
dc.contributor.author | Wang, Sheng | en_US |
dc.contributor.author | Sun, Hao | en_US |
dc.contributor.author | Tao, Yile | en_US |
dc.contributor.author | Sun, Yujie | en_US |
dc.date.accessioned | 2014-09-08T15:36:35Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Liu, Z., D. Xing, Q. P. Su, Y. Zhu, J. Zhang, X. Kong, B. Xue, et al. 2014. “Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space.” Nature Communications 5 (1): 4443. doi:10.1038/ncomms5443. http://dx.doi.org/10.1038/ncomms5443. | en |
dc.identifier.issn | 2041-1723 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785855 | |
dc.description.abstract | Imaging the location and dynamics of individual interacting protein pairs is essential but often difficult because of the fluorescent background from other paired and non-paired molecules, particularly in the sub-diffraction cellular space. Here we develop a new method combining bimolecular fluorescence complementation and photoactivated localization microscopy for super-resolution imaging and single-molecule tracking of specific protein–protein interactions. The method is used to study the interaction of two abundant proteins, MreB and EF-Tu, in Escherichia coli cells. The super-resolution imaging shows interesting distribution and domain sizes of interacting MreB–EF-Tu pairs as a subpopulation of total EF-Tu. The single-molecule tracking of MreB, EF-Tu and MreB–EF-Tu pairs reveals intriguing localization-dependent heterogonous dynamics and provides valuable insights to understanding the roles of MreB–EF-Tu interactions. | en |
dc.language.iso | en_US | en |
dc.publisher | Nature Pub. Group | en |
dc.relation.isversionof | doi:10.1038/ncomms5443 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4109008/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Nature Communications | en |
dash.depositing.author | Xing, Dong | en_US |
dc.date.available | 2014-09-08T15:36:35Z | |
dc.identifier.doi | 10.1038/ncomms5443 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Xing, Dong | |