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dc.contributor.authorDephoure, Noahen_US
dc.contributor.authorHwang, Sunyoungen_US
dc.contributor.authorO'Sullivan, Ciaraen_US
dc.contributor.authorDodgson, Stacie Een_US
dc.contributor.authorGygi, Steven Pen_US
dc.contributor.authorAmon, Angelikaen_US
dc.contributor.authorTorres, Eduardo Men_US
dc.date.accessioned2014-09-08T15:36:55Z
dc.date.issued2014en_US
dc.identifier.citationDephoure, Noah, Sunyoung Hwang, Ciara O'Sullivan, Stacie E Dodgson, Steven P Gygi, Angelika Amon, and Eduardo M Torres. 2014. “Quantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeast.” eLife 3 (1): e03023. doi:10.7554/eLife.03023. http://dx.doi.org/10.7554/eLife.03023.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12785881
dc.description.abstractAneuploidy causes severe developmental defects and is a near universal feature of tumor cells. Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. We show that although protein levels largely scale with gene copy number, subunits of multi-protein complexes are notable exceptions. Posttranslational mechanisms attenuate their expression when their encoding genes are in excess. Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. Indeed aneuploid cells harbor increased levels of reactive oxygen species (ROS). Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. Cells respond to these alterations through both transcriptional and posttranscriptional mechanisms. DOI: http://dx.doi.org/10.7554/eLife.03023.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.03023en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129440/pdf/en
dash.licenseLAAen_US
dc.subjectaneuploidyen
dc.subjectposttranslational mechanismsen
dc.subjectposttranscriptional mechanismsen
dc.subjectproteomicsen
dc.subjecten
dc.titleQuantitative proteomic analysis reveals posttranslational responses to aneuploidy in yeasten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorDephoure, Noahen_US
dc.date.available2014-09-08T15:36:55Z
dc.identifier.doi10.7554/eLife.03023*
dash.contributor.affiliatedDephoure, Noah Elias
dash.contributor.affiliatedGygi, Steven


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