Protein interaction mapping with ribosome-displayed using PLATO ORF libraries
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Author
Zhu, Jian
Larman, H. Benjamin
Gao, Geng
Somwar, Romel
Zhang, Zijuan
Laserson, Uri
Ciccia, Alberto
Pavlova, Natalya
Zhang, Wei
Kesari, Santosh
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/nprot.2013.167Metadata
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Zhu, J., H. B. Larman, G. Gao, R. Somwar, Z. Zhang, U. Laserson, A. Ciccia, et al. 2013. “Protein interaction mapping with ribosome-displayed using PLATO ORF libraries.” Nature protocols 9 (1): 90-103. doi:10.1038/nprot.2013.167. http://dx.doi.org/10.1038/nprot.2013.167.Abstract
Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method by identifying known and new interacting partners of LYN kinase, patient autoantibodies and the small molecules gefitinib and dasatinib.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129458/pdf/Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785882
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