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dc.contributor.authorKaragiannides, Iordanesen_US
dc.contributor.authorGolovatscka, Viktoriyaen_US
dc.contributor.authorBakirtzi, Kyriakien_US
dc.contributor.authorSideri, Aristeaen_US
dc.contributor.authorSalas, Marthaen_US
dc.contributor.authorStavrakis, Dimitrisen_US
dc.contributor.authorPolytarchou, Christosen_US
dc.contributor.authorIliopoulos, Dimitriosen_US
dc.contributor.authorPothoulakis, Charalabosen_US
dc.contributor.authorBradesi, Sylvieen_US
dc.date.accessioned2014-09-08T15:37:19Z
dc.date.issued2014en_US
dc.identifier.citationKaragiannides, Iordanes, Viktoriya Golovatscka, Kyriaki Bakirtzi, Aristea Sideri, Martha Salas, Dimitris Stavrakis, Christos Polytarchou, Dimitrios Iliopoulos, Charalabos Pothoulakis, and Sylvie Bradesi. 2014. “Chronic unpredictable stress regulates visceral adipocyte‐mediated glucose metabolism and inflammatory circuits in male rats.” Physiological Reports 2 (5): e00284. doi:10.14814/phy2.284. http://dx.doi.org/10.14814/phy2.284.en
dc.identifier.issn2051-817Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12785925
dc.description.abstractAbstract Chronic psychological stress is a prominent risk factor involved in the pathogenesis of many complex diseases, including major depression, obesity, and type II diabetes. Visceral adipose tissue is a key endocrine organ involved in the regulation of insulin action and an important component in the development of insulin resistance. Here, we examined for the first time the changes on visceral adipose tissue physiology and on adipocyte‐associated insulin sensitivity and function after chronic unpredictable stress in rats. Male rats were subjected to chronic unpredictable stress for 35 days. Total body and visceral fat was measured. Cytokines and activated intracellular kinase levels were determined using high‐throughput multiplex assays. Adipocyte function was assessed via tritiated glucose uptake assay. Stressed rats showed no weight gain, and their fat/lean mass ratio increased dramatically compared to control animals. Stressed rats had significantly higher mesenteric fat content and epididymal fat pad weight and demonstrated reduced serum glucose clearing capacity following glucose challenge. Alterations in fat depot size were mainly due to changes in adipocyte numbers and not size. High‐throughput molecular screening in adipocytes isolated from stressed rats revealed activation of intracellular inflammatory, glucose metabolism, and MAPK networks compared to controls, as well as significantly reduced glucose uptake capacity in response to insulin stimulation. Our study identifies the adipocyte as a key regulator of the effects of chronic stress on insulin resistance, and glucose metabolism, with important ramifications in the pathophysiology of several stress‐related disease states.en
dc.language.isoen_USen
dc.publisherWiley Periodicals, Inc.en
dc.relation.isversionofdoi:10.14814/phy2.284en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4098730/pdf/en
dash.licenseLAAen_US
dc.subjectAdipocytesen
dc.subjectadipokinesen
dc.subjectfat tissueen
dc.subjectinsulin resistanceen
dc.subjectstressen
dc.titleChronic unpredictable stress regulates visceral adipocyte‐mediated glucose metabolism and inflammatory circuits in male ratsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPhysiological Reportsen
dc.date.available2014-09-08T15:37:19Z
dc.identifier.doi10.14814/phy2.284*


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