dc.contributor.author | Zhou, Xiaohui | en_US |
dc.contributor.author | Massol, Ramiro H. | en_US |
dc.contributor.author | Nakamura, Fumihiko | en_US |
dc.contributor.author | Chen, Xiang | en_US |
dc.contributor.author | Gewurz, Benjamin E. | en_US |
dc.contributor.author | Davis, Brigid M. | en_US |
dc.contributor.author | Lencer, Wayne I. | en_US |
dc.contributor.author | Waldor, Matthew K. | en_US |
dc.date.accessioned | 2014-09-08T15:37:23Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Zhou, Xiaohui, Ramiro H. Massol, Fumihiko Nakamura, Xiang Chen, Benjamin E. Gewurz, Brigid M. Davis, Wayne I. Lencer, and Matthew K. Waldor. 2014. “Remodeling of the Intestinal Brush Border Underlies Adhesion and Virulence of an Enteric Pathogen.” mBio 5 (4): e01639-14. doi:10.1128/mBio.01639-14. http://dx.doi.org/10.1128/mBio.01639-14. | en |
dc.identifier.issn | 2150-7511 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:12785933 | |
dc.description.abstract | ABSTRACT Intestinal colonization by Vibrio parahaemolyticus—the most common cause of seafood-borne bacterial enteritis worldwide—induces extensive disruption of intestinal microvilli. In orogastrically infected infant rabbits, reorganization of the apical brush border membrane includes effacement of some microvilli and marked elongation of others. All diarrhea, inflammation, and intestinal pathology associated with V. parahaemolyticus infection are dependent upon one of its type 3 secretion systems (T3SS2); however, translocated effectors that directly mediate brush border restructuring and bacterial adhesion are not known. Here, we demonstrate that the effector VopV is essential for V. parahaemolyticus intestinal colonization and therefore its pathogenicity, that it induces effacement of brush border microvilli, and that this effacement is required for adhesion of V. parahaemolyticus to enterocytes. VopV contains multiple functionally independent and mechanistically distinct domains through which it disrupts microvilli. We show that interaction between VopV and filamin, as well as VopV’s previously noted interaction with actin, mediates enterocyte cytoskeletal reorganization. VopV’s multipronged approach to epithelial restructuring, coupled with its impact on colonization, suggests that remodeling of the epithelial brush border is a critical step in pathogenesis. | en |
dc.language.iso | en_US | en |
dc.publisher | American Society of Microbiology | en |
dc.relation.isversionof | doi:10.1128/mBio.01639-14 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147867/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Remodeling of the Intestinal Brush Border Underlies Adhesion and Virulence of an Enteric Pathogen | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | mBio | en |
dash.depositing.author | Zhou, Xiaohui | en_US |
dc.date.available | 2014-09-08T15:37:23Z | |
dc.identifier.doi | 10.1128/mBio.01639-14 | * |
dash.contributor.affiliated | Zhou, Xiaohui | |
dash.contributor.affiliated | Nakamura, Fumihiko | |