Two Antagonistic Clock-Regulated Histidine Kinases Time the Activation of Circadian Gene Expression

DSpace/Manakin Repository

Two Antagonistic Clock-Regulated Histidine Kinases Time the Activation of Circadian Gene Expression

Citable link to this page

 

 
Title: Two Antagonistic Clock-Regulated Histidine Kinases Time the Activation of Circadian Gene Expression
Author: Gutu, Andrian; O'Shea, Erin K.

Note: Order does not necessarily reflect citation order of authors.

Citation: Gutu, Andrian, and Erin K. O'Shea. 2013. Two Antagonistic Clock-Regulated Histidine Kinases Time the Activation of Circadian Gene Expression. Molecular Cell 50, no. 2: 288–294.
Full Text & Related Files:
Abstract: The cyanobacterial circadian pacemaker consists of a three-protein clock—KaiA, KaiB, and KaiC—that generates oscillations in the phosphorylation state of KaiC. Here we investigate how temporal information encoded in KaiC phosphorylation is transduced to RpaA, a transcription factor required for circadian gene expression. We show that phosphorylation of RpaA is regulated by two antagonistic histidine kinases, SasA and CikA, which are sequentially activated at distinct times by the Kai clock complex. SasA acts as a kinase toward RpaA, whereas CikA, previously implicated in clock input, acts as a phosphatase that dephosphorylates RpaA. CikA and SasA cooperate to generate an oscillation of RpaA activity that is distinct from that generated by either enzyme alone and offset from the rhythm of KaiC phosphorylation. Our observations reveal how circadian clocks can precisely control the timing of output pathways via the concerted action of two oppositely acting enzymes.
Published Version: doi:10.1016/j.molcel.2013.02.022
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3674810/
https://www.mcb.harvard.edu/mcb_files/media/editor_uploads/2013/03/1-s2.0-S1097276513001780-main-1.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:12872189
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters