Bright and Fast Multicoloured Voltage Reporters via Electrochromic FRET
Douglass, Adam D.
Harrison, D. Jed
Campbell, Robert E.
MetadataShow full item record
CitationZou, Peng, Yongxin Zhao, Adam D. Douglass, Daniel R. Hochbaum, Daan Brinks, Christopher A. Werley, D. Jed Harrison, Robert E. Campbell, and Adam E. Cohen. 2014. Bright and Fast Multicoloured Voltage Reporters via Electrochromic FRET. Nature Communications 5 (August 13): 4625.
AbstractGenetically encoded fluorescent reporters of membrane potential promise to reveal aspects of neural function not detectable by other means. We present a palette of multicoloured brightly fluorescent genetically encoded voltage indicators with sensitivities from 8–13% ΔF/F per 100 mV, and half-maximal response times from 4–7 ms. A fluorescent protein is fused to an archaerhodopsin-derived voltage sensor. Voltage-induced shifts in the absorption spectrum of the rhodopsin lead to voltage-dependent nonradiative quenching of the appended fluorescent protein. Through a library screen, we identify linkers and fluorescent protein combinations that report neuronal action potentials in cultured rat hippocampal neurons with a single-trial signal-to-noise ratio from 7 to 9 in a 1 kHz imaging bandwidth at modest illumination intensity. The freedom to choose a voltage indicator from an array of colours facilitates multicolour voltage imaging, as well as combination with other optical reporters and optogenetic actuators.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12872204
- FAS Scholarly Articles