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dc.contributor.authorNayak, Dipti D.en_US
dc.contributor.authorMarx, Christopher J.en_US
dc.date.accessioned2014-10-01T14:28:27Z
dc.date.issued2014en_US
dc.identifier.citationNayak, Dipti D., and Christopher J. Marx. 2014. “Genetic and Phenotypic Comparison of Facultative Methylotrophy between Methylobacterium extorquens Strains PA1 and AM1.” PLoS ONE 9 (9): e107887. doi:10.1371/journal.pone.0107887. http://dx.doi.org/10.1371/journal.pone.0107887.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12987289
dc.description.abstractMethylobacterium extorquens AM1, a strain serendipitously isolated half a century ago, has become the best-characterized model system for the study of aerobic methylotrophy (the ability to grow on reduced single-carbon compounds). However, with 5 replicons and 174 insertion sequence (IS) elements in the genome as well as a long history of domestication in the laboratory, genetic and genomic analysis of M. extorquens AM1 face several challenges. On the contrary, a recently isolated strain - M. extorquens PA1- is closely related to M. extorquens AM1 (100% 16S rRNA identity) and contains a streamlined genome with a single replicon and only 20 IS elements. With the exception of the methylamine dehydrogenase encoding gene cluster (mau), genes known to be involved in methylotrophy are well conserved between M. extorquens AM1 and M. extorquens PA1. In this paper we report four primary findings regarding methylotrophy in PA1. First, with a few notable exceptions, the repertoire of methylotrophy genes between PA1 and AM1 is extremely similar. Second, PA1 grows faster with higher yields compared to AM1 on C1 and multi-C substrates in minimal media, but AM1 grows faster in rich medium. Third, deletion mutants in PA1 throughout methylotrophy modules have the same C1 growth phenotypes observed in AM1. Finally, the precision of our growth assays revealed several unexpected growth phenotypes for various knockout mutants that serve as leads for future work in understanding their basis and generality across Methylobacterium strains.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0107887en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169470/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectGeneticsen
dc.subjectGenomicsen
dc.subjectMicrobial Genomicsen
dc.subjectBacterial Genomicsen
dc.subjectMicrobiologyen
dc.subjectBacteriologyen
dc.subjectBacterial Genesen
dc.subjectBacterial Physiologyen
dc.subjectBacterial Taxonomyen
dc.subjectGram Negative Bacteriaen
dc.subjectMicrobial Physiologyen
dc.subjectMicrobial Metabolismen
dc.titleGenetic and Phenotypic Comparison of Facultative Methylotrophy between Methylobacterium extorquens Strains PA1 and AM1en
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorMarx, Christopher J.en_US
dc.date.available2014-10-01T14:28:27Z
dc.identifier.doi10.1371/journal.pone.0107887*
dash.contributor.affiliatedMarx, Christopher J


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