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dc.contributor.authorLeite, Luciana Ferreiraen_US
dc.contributor.authorGomez, Renato Santiagoen_US
dc.contributor.authorFonseca, Matheus de Castroen_US
dc.contributor.authorGomez, Marcus Viniciusen_US
dc.contributor.authorGuatimosim, Cristinaen_US
dc.date.accessioned2014-10-01T14:28:28Z
dc.date.issued2011en_US
dc.identifier.citationLeite, Luciana Ferreira, Renato Santiago Gomez, Matheus de Castro Fonseca, Marcus Vinicius Gomez, and Cristina Guatimosim. 2011. “Effect of intravenous anesthetic propofol on synaptic vesicle exocytosis at the frog neuromuscular junction.” Acta Pharmacologica Sinica 32 (1): 31-37. doi:10.1038/aps.2010.175. http://dx.doi.org/10.1038/aps.2010.175.en
dc.identifier.issn1671-4083en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12987290
dc.description.abstractAim: To investigate the presynaptic effects of propofol, a short-acting intravenous anesthetic, in the frog neuromuscular junction. Methods: Frog cutaneous pectoris nerve muscle preparations were prepared. A fluorescent tool (FM1-43) was used to visualize the effect of propofol on synaptic vesicle exocytosos in the frog neuromuscular junction. Results: Low concentrations of propofol, ranging from 10 to 25 μmol/L, enhanced spontaneous vesicle exocytosis monitored by FM1-43 in a Ca2+-dependent and Na+-independent fashion. Higher concentrations of propofol (50, 100, and 200 μmol/L) had no effect on spontaneous exocytosis. By contrast, higher concentrations of propofol inhibited the Na+-dependent exocytosis evoked by 4-aminopyridine but did not affect the Na+-independent exocytosis evoked by KCl. This action was similar and non-additive with that observed by tetrodotoxin, a Na+ channel blocker. Conclusion: Our data suggest that propofol has a dose-dependent presynaptic effect at the neuromuscular transmission which may help to understand some of the clinical effects of this agent on neuromuscular function.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/aps.2010.175en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003312/pdf/en
dash.licenseLAAen_US
dc.subjectpropofolen
dc.subjectneuromuscular junctionen
dc.subjectfrogen
dc.subjectFM1-43en
dc.subjectsynaptic vesicleen
dc.subjectgeneral anestheticen
dc.titleEffect of intravenous anesthetic propofol on synaptic vesicle exocytosis at the frog neuromuscular junctionen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalActa Pharmacologica Sinicaen
dc.date.available2014-10-01T14:28:28Z
dc.identifier.doi10.1038/aps.2010.175*
dash.authorsorderedfalse


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