Show simple item record

dc.contributor.authorHasegawa, Eiichien_US
dc.contributor.authorSweigard, Harryen_US
dc.contributor.authorHusain, Deebaen_US
dc.contributor.authorOlivares, Ana M.en_US
dc.contributor.authorChang, Boen_US
dc.contributor.authorSmith, Kaylee E.en_US
dc.contributor.authorBirsner, Amy E.en_US
dc.contributor.authorD’Amato, Robert J.en_US
dc.contributor.authorMichaud, Norman A.en_US
dc.contributor.authorHan, Yinanen_US
dc.contributor.authorVavvas, Demetrios G.en_US
dc.contributor.authorMiller, Joan W.en_US
dc.contributor.authorHaider, Neena B.en_US
dc.contributor.authorConnor, Kip M.en_US
dc.date.accessioned2014-10-01T14:28:38Z
dc.date.issued2014en_US
dc.identifier.citationHasegawa, E., H. Sweigard, D. Husain, A. M. Olivares, B. Chang, K. E. Smith, A. E. Birsner, et al. 2014. “Characterization of a Spontaneous Retinal Neovascular Mouse Model.” PLoS ONE 9 (9): e106507. doi:10.1371/journal.pone.0106507. http://dx.doi.org/10.1371/journal.pone.0106507.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12987300
dc.description.abstractBackground: Vision loss due to vascular disease of the retina is a leading cause of blindness in the world. Retinal angiomatous proliferation (RAP) is a subgroup of neovascular age-related macular degeneration (AMD), whereby abnormal blood vessels develop in the retina leading to debilitating vision loss and eventual blindness. The novel mouse strain, neoretinal vascularization 2 (NRV2), shows spontaneous fundus changes associated with abnormal neovascularization. The purpose of this study is to characterize the induction of pathologic angiogenesis in this mouse model. Methods: The NRV2 mice were examined from postnatal day 12 (p12) to 3 months. The phenotypic changes within the retina were evaluated by fundus photography, fluorescein angiography, optical coherence tomography, and immunohistochemical and electron microscopic analysis. The pathological neovascularization was imaged by confocal microscopy and reconstructed using three-dimensional image analysis software. Results: We found that NRV2 mice develop multifocal retinal depigmentation in the posterior fundus. Depigmented lesions developed vascular leakage observed by fluorescein angiography. The spontaneous angiogenesis arose from the retinal vascular plexus at postnatal day (p)15 and extended toward retinal pigment epithelium (RPE). By three months of age, histological analysis revealed encapsulation of the neovascular lesion by the RPE in the photoreceptor cell layer and subretinal space. Conclusions: The NRV2 mouse strain develops early neovascular lesions within the retina, which grow downward towards the RPE beginning at p15. This retinal neovascularization model mimics early stages of human retinal angiomatous proliferation (RAP) and will likely be a useful in elucidating targeted therapeutics for patients with ocular neovascular disease.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0106507en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154693/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectCell Biologyen
dc.subjectDevelopmental Biologyen
dc.subjectMedicine and Health Sciencesen
dc.subjectOphthalmologyen
dc.subjectEye Diseasesen
dc.subjectMacular Disordersen
dc.subjectRetinal Disordersen
dc.subjectVisual Impairmentsen
dc.subjectAnimal Studiesen
dc.subjectAnimal Models of Diseaseen
dc.titleCharacterization of a Spontaneous Retinal Neovascular Mouse Modelen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorHasegawa, Eiichien_US
dc.date.available2014-10-01T14:28:38Z
dc.identifier.doi10.1371/journal.pone.0106507*
dash.authorsorderedfalse
dash.contributor.affiliatedHasegawa, Eiichi
dash.contributor.affiliatedHaider, Neena
dash.contributor.affiliatedConnor, Kip
dash.contributor.affiliatedHusain, Deeba
dash.contributor.affiliatedMiller, Joan
dash.contributor.affiliatedVavvas, Demetrios


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record