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dc.contributor.authorLarman, H. Benjaminen_US
dc.contributor.authorZhao, Zhenmingen_US
dc.contributor.authorLaserson, Urien_US
dc.contributor.authorLi, Mamie Z.en_US
dc.contributor.authorCiccia, Albertoen_US
dc.contributor.authorGakidis, M. Angelica Martinezen_US
dc.contributor.authorChurch, George M.en_US
dc.contributor.authorKesari, Santoshen_US
dc.contributor.authorLeProust, Emily M.en_US
dc.contributor.authorSolimini, Nicole L.en_US
dc.contributor.authorElledge, Stephen J.en_US
dc.date.accessioned2014-10-01T14:28:59Z
dc.date.issued2011en_US
dc.identifier.citationLarman, H. B., Z. Zhao, U. Laserson, M. Z. Li, A. Ciccia, M. A. M. Gakidis, G. M. Church, et al. 2011. “Application of a synthetic human proteome to autoantigen discovery through PhIP-Seq.” Nature biotechnology 29 (6): 535-541. doi:10.1038/nbt.1856. http://dx.doi.org/10.1038/nbt.1856.en
dc.identifier.issn1087-0156en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12987341
dc.description.abstractIn this study, we improve on current autoantigen discovery approaches by creating a synthetic representation of the complete human proteome, the T7 “peptidome” phage display library (T7-Pep), and use it to profile the autoantibody repertoires of individual patients. We provide methods for 1) designing and cloning large libraries of DNA microarray-derived oligonucleotides encoding peptides for display on bacteriophage, and 2) analysis of the peptide libraries using high throughput DNA sequencing. We applied phage immunoprecipitation sequencing (PhIP-Seq) to identify both known and novel autoantibodies contained in the spinal fluid of three patients with paraneoplastic neurological syndromes. We also show how our approach can be used more generally to identify peptide-protein interactions and point toward ways in which this technology will be further developed in the future. We envision that PhIP-Seq can become an important new tool in autoantibody analysis, as well as proteomic research in general.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nbt.1856en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4169279/pdf/en
dash.licenseLAAen_US
dc.subjectSynthetic biologyen
dc.subjectproteomicsen
dc.subjectphage displayen
dc.subjecthumoral autoimmunityen
dc.subjectparaneoplastic neurological disorderen
dc.subjectprotein-protein interactionsen
dc.titleApplication of a synthetic human proteome to autoantigen discovery through PhIP-Seqen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature biotechnologyen
dash.depositing.authorChurch, George M.en_US
dc.date.available2014-10-01T14:28:59Z
dc.identifier.doi10.1038/nbt.1856*
dash.authorsorderedfalse
dash.contributor.affiliatedElledge, Stephen
dash.contributor.affiliatedChurch, George


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