Show simple item record

dc.contributor.authorGu, Yuanen_US
dc.contributor.authorYuan, Jun-yingen_US
dc.contributor.authorSun, Xiao-xiaoen_US
dc.contributor.authorYe, Ji-mingen_US
dc.contributor.authorHe, Lien_US
dc.contributor.authorYan, Shou-shengen_US
dc.contributor.authorZhang, Hao-haoen_US
dc.contributor.authorHu, Li-hongen_US
dc.contributor.authorYu, Qiangen_US
dc.date.accessioned2014-10-01T14:29:13Z
dc.date.issued2012en_US
dc.identifier.citationGu, Yuan, Xiao-xiao Sun, Ji-ming Ye, Li He, Shou-sheng Yan, Hao-hao Zhang, Li-hong Hu, Jun-ying Yuan, and Qiang Yu. 2012. “Arctigenin alleviates ER stress via activating AMPK.” Acta Pharmacologica Sinica 33 (7): 941-952. doi:10.1038/aps.2012.60. http://dx.doi.org/10.1038/aps.2012.60.en
dc.identifier.issn1671-4083en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:12987366
dc.description.abstractAim: To investigate the protective effects of arctigenin (ATG), a phenylpropanoid dibenzylbutyrolactone lignan from Arctium lappa L (Compositae), against ER stress in vitro and the underlying mechanisms. Methods: A cell-based screening assay for ER stress regulators was established. Cell viability was measured using MTT assay. PCR and Western blotting were used to analyze gene and protein expression. Silencing of the CaMKKβ, LKB1, and AMPKα1 genes was achieved by RNA interference (RNAi). An ATP bioluminescent assay kit was employed to measure the intracellular ATP levels. Results: ATG (2.5, 5 and 10 μmol/L) inhibited cell death and unfolded protein response (UPR) in a concentration-dependent manner in cells treated with the ER stress inducer brefeldin A (100 nmol/L). ATG (1, 5 and 10 μmol/L) significantly attenuated protein synthesis in cells through inhibiting mTOR-p70S6K signaling and eEF2 activity, which were partially reversed by silencing AMPKα1 with RNAi. ATG (1-50 μmol/L) reduced intracellular ATP level and activated AMPK through inhibiting complex I-mediated respiration. Pretreatment of cells with the AMPK inhibitor compound C (25 μmol/L) rescued the inhibitory effects of ATG on ER stress. Furthermore, ATG (2.5 and 5 μmol/L) efficiently activated AMPK and reduced the ER stress and cell death induced by palmitate (2 mmol/L) in INS-1 β cells. Conclusion: ATG is an effective ER stress alleviator, which protects cells against ER stress through activating AMPK, thus attenuating protein translation and reducing ER load.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/aps.2012.60en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011149/pdf/en
dash.licenseLAAen_US
dc.subjectarctigeninen
dc.subjectER stressen
dc.subjecthuman hepatocellular liver carcinoma cellen
dc.subjectβ-cell deathen
dc.subjectmTOR-p70S6Ken
dc.subjecteukaryotic translation elongation factor 2 (eEF2)en
dc.subjectmitochondrial respirationen
dc.subjectAMPKen
dc.titleArctigenin alleviates ER stress via activating AMPKen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalActa Pharmacologica Sinicaen
dc.date.available2014-10-01T14:29:13Z
dc.identifier.doi10.1038/aps.2012.60*


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record