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dc.contributor.authorWhiteman, Noah K.
dc.contributor.authorGroen, Simon C.
dc.contributor.authorChevasco, Daniela
dc.contributor.authorBear, Ashley
dc.contributor.authorBeckwith, Noor M R
dc.contributor.authorGregory, T. Ryan
dc.contributor.authorDenoux, Carine
dc.contributor.authorMammarella, Nicole
dc.contributor.authorAusubel, Frederick M.
dc.contributor.authorPierce, Naomi E.
dc.date.accessioned2014-10-14T19:49:36Z
dc.date.issued2010
dc.identifier.citationWhiteman, Noah K., Simon C. Groen, Daniela Chevasco, Ashley Bear, Noor Beckwith, T. Ryan Gregory, Carine Denoux, Nicole Mammarella, Frederick M. Ausubel, and Naomi E. Pierce. 2010. Mining the Plant-Herbivore Interface with a Leafmining Drosophila of Arabidopsis. Molecular Ecology 20, no. 5: 995–1014.en_US
dc.identifier.issn0962-1083en_US
dc.identifier.issn1365-294Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13041322
dc.description.abstractExperimental infections of Arabidopsis thaliana (Arabidopsis) with genomically characterized plant pathogens such as Pseudomonas syringae have facilitated the dissection of canonical eukaryotic defence pathways and parasite virulence factors. Plants are also attacked by herbivorous insects, and the development of an ecologically relevant genetic model herbivore that feeds on Arabidopsis will enable the parallel dissection of host defence and reciprocal resistance pathways such as those involved in xenobiotic metabolism. An ideal candidate is Scaptomyza flava, a drosophilid fly whose leafmining larvae are true herbivores that can be found in nature feeding on Arabidopsis and other crucifers. Here, we describe the life cycle of S. flava on Arabidopsis and use multiple approaches to characterize the response of Arabidopsis to S. flava attack. Oviposition choice tests and growth performance assays on different Arabidopsis ecotypes, defence-related mutants, and hormone and chitin-treated plants revealed significant differences in host preference and variation in larval performance across Arabidopsis accessions. The jasmonate and glucosinolate pathways in Arabidopsis are important in mediating quantitative resistance against S. flava, and priming with jasmonate or chitin resulted in increased resistance. Expression of xenobiotic detoxification genes was reduced in S. flava larvae reared on Arabidopsis jasmonate signalling mutants and increased in plants pretreated with chitin. These results and future research directions are discussed in the context of developing a genetic model system to analyse insect–plant interactions.en_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dc.relation.isversionofdoi:10.1111/j.1365-294X.2010.04901.xen_US
dc.relation.hasversionhttp://www.oeb.harvard.edu/faculty/pierce/publications/pdfs/2011_Whiteman_et_al_Scaptomyza.pdfen_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062943/pdf/nihms246790.pdfen_US
dash.licenseOAP
dc.subjectArabidopsisen_US
dc.subjectDrosophilaen_US
dc.subjectgene expressionen_US
dc.subjectherbivoryen_US
dc.subjectjasmonateen_US
dc.subjectmodel systemen_US
dc.titleMining the Plant-Herbivore Interface with a Leafmining Drosophila of Arabidopsisen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalMolecular Ecologyen_US
dash.depositing.authorPierce, Naomi E.
dc.date.available2014-10-14T19:49:36Z
dash.affiliation.otherHarvard Medical Schoolen_US
dc.identifier.doi10.1111/j.1365-294X.2010.04901.x*
workflow.legacycommentsFAR 2012; manuscript version retrieved from NIH (see link in other available sources)en_US
dash.contributor.affiliatedAusubel, Frederick
dash.contributor.affiliatedBeckwith, Noor
dash.contributor.affiliatedPierce, Naomi


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