Small-Molecule Screen Identifies Reactive Oxygen Species as Key Regulators of Neutrophil Chemotaxis
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Author
Hattori, Hidenori
Subramanian, Kulandayan K.
Sakai, Jiro
Jia, Yonghui
Li, Yitang
Porter, Timothy F.
Sarraj, Bara
Kasorn, Anongnard
Blanchard, Catlyn
Zirkle, Dorothy
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1073/pnas.0914351107Metadata
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Hattori, Hidenori, Kulandayan K. Subramanian, Jiro Sakai, Yonghui Jia, Yitang Li, Timothy F. Porter, Fabien Loison, et al. 2010. “Small-Molecule Screen Identifies Reactive Oxygen Species as Key Regulators of Neutrophil Chemotaxis.” Proceedings of the National Academy of Sciences 107 (8) (February 8): 3546–3551. doi:10.1073/pnas.0914351107. http://dx.doi.org/10.1073/pnas.0914351107.Abstract
Neutrophil chemotaxis plays an essential role in innate immunity, but the underlying cellular mechanism is still not fully characterized. Here, using a small-molecule functional screening, we identified NADPH oxidase–dependent reactive oxygen species as key regulators of neutrophil chemotactic migration. Neutrophils with pharmacologically inhibited oxidase, or isolated from chronic granulomatous disease (CGD) patients and mice, formed more frequent multiple pseudopodia and lost their directionality as they migrated up a chemoattractant concentration gradient. Knocking down NADPH oxidase in differentiated neutrophil-like HL60 cells also led to defective chemotaxis. Consistent with the in vitro results, adoptively transferred CGD murine neutrophils showed impaired in vivo recruitment to sites of inflammation. Together, these results present a physiological role for reactive oxygen species in regulating neutrophil functions and shed light on the pathogenesis of CGD.Other Sources
http://www.ncbi.nlm.nih.gov/pubmed/20142487Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:13320257
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