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dc.contributor.authorHou, Jennifer H.en_US
dc.contributor.authorKralj, Joel M.en_US
dc.contributor.authorDouglass, Adam D.en_US
dc.contributor.authorEngert, Florianen_US
dc.contributor.authorCohen, Adam E.en_US
dc.date.accessioned2014-11-03T17:39:15Z
dc.date.issued2014en_US
dc.identifier.citationHou, Jennifer H., Joel M. Kralj, Adam D. Douglass, Florian Engert, and Adam E. Cohen. 2014. “Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents.” Frontiers in Physiology 5 (1): 344. doi:10.3389/fphys.2014.00344. http://dx.doi.org/10.3389/fphys.2014.00344.en
dc.identifier.issn1664-042Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347493
dc.description.abstractThe cardiac action potential (AP) and the consequent cytosolic Ca2+ transient are key indicators of cardiac function. Natural developmental processes, as well as many drugs and pathologies change the waveform, propagation, or variability (between cells or over time) of these parameters. Here we apply a genetically encoded dual-function calcium and voltage reporter (CaViar) to study the development of the zebrafish heart in vivo between 1.5 and 4 days post fertilization (dpf). We developed a high-sensitivity spinning disk confocal microscope and associated software for simultaneous three-dimensional optical mapping of voltage and calcium. We produced a transgenic zebrafish line expressing CaViar under control of the heart-specific cmlc2 promoter, and applied ion channel blockers at a series of developmental stages to map the maturation of the action potential in vivo. Early in development, the AP initiated via a calcium current through L-type calcium channels. Between 90 and 102 h post fertilization (hpf), the ventricular AP switched to a sodium-driven upswing, while the atrial AP remained calcium driven. In the adult zebrafish heart, a sodium current drives the AP in both the atrium and ventricle. Simultaneous voltage and calcium imaging with genetically encoded reporters provides a new approach for monitoring cardiac development, and the effects of drugs on cardiac function.en
dc.language.isoen_USen
dc.publisherFrontiers Media S.A.en
dc.relation.isversionofdoi:10.3389/fphys.2014.00344en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161048/pdf/en
dash.licenseLAAen_US
dc.subjectvoltage imagingen
dc.subjectcardiac developmenten
dc.titleSimultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currentsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalFrontiers in Physiologyen
dash.depositing.authorEngert, Florianen_US
dc.date.available2014-11-03T17:39:15Z
dc.identifier.doi10.3389/fphys.2014.00344*
dash.contributor.affiliatedCohen, Adam
dash.contributor.affiliatedEngert, Florian


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