In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells

DSpace/Manakin Repository

In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells

Citable link to this page


Title: In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells
Author: Malka, Roy; Delgado, Francisco Feijó; Manalis, Scott R.; Higgins, John M.

Note: Order does not necessarily reflect citation order of authors.

Citation: Malka, Roy, Francisco Feijó Delgado, Scott R. Manalis, and John M. Higgins. 2014. “In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells.” PLoS Computational Biology 10 (10): e1003839. doi:10.1371/journal.pcbi.1003839.
Full Text & Related Files:
Abstract: Human red blood cells (RBCs) lose ∼30% of their volume and ∼20% of their hemoglobin (Hb) content during their ∼100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing vesicles during their life in the circulation, and this process is thought to dominate the changes in the RBC physical characteristics occurring during maturation. We combine theory with single-cell measurements to investigate the impact of vesiculation on the reduction in volume, Hb mass, and membrane. We show that vesicle shedding alone is sufficient to explain membrane losses but not volume or Hb losses. We use dry mass measurements of human RBCs to validate the models and to propose that additional unknown mechanisms control volume and Hb reduction and are responsible for ∼90% of the observed reduction. RBC population characteristics are used in the clinic to monitor and diagnose a wide range of conditions including malnutrition, inflammation, and cancer. Quantitative characterization of cellular maturation processes may help in the early detection of clinical conditions where maturation patterns are altered.
Published Version: doi:10.1371/journal.pcbi.1003839
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search