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dc.contributor.authorGelfand, Maria Ven_US
dc.contributor.authorHagan, Nellwynen_US
dc.contributor.authorTata, Aleksandraen_US
dc.contributor.authorOh, Won-Jongen_US
dc.contributor.authorLacoste, Baptisteen_US
dc.contributor.authorKang, Kyu-Taeen_US
dc.contributor.authorKopycinska, Justynaen_US
dc.contributor.authorBischoff, Joyceen_US
dc.contributor.authorWang, Jia-Huaien_US
dc.contributor.authorGu, Chenghuaen_US
dc.date.accessioned2014-11-03T17:39:54Z
dc.date.issued2014en_US
dc.identifier.citationGelfand, Maria V, Nellwyn Hagan, Aleksandra Tata, Won-Jong Oh, Baptiste Lacoste, Kyu-Tae Kang, Justyna Kopycinska, Joyce Bischoff, Jia-Huai Wang, and Chenghua Gu. 2014. “Neuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand binding.” eLife 3 (1): e03720. doi:10.7554/eLife.03720. http://dx.doi.org/10.7554/eLife.03720.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347555
dc.description.abstractDuring development, tissue repair, and tumor growth, most blood vessel networks are generated through angiogenesis. Vascular endothelial growth factor (VEGF) is a key regulator of this process and currently both VEGF and its receptors, VEGFR1, VEGFR2, and Neuropilin1 (NRP1), are targeted in therapeutic strategies for vascular disease and cancer. NRP1 is essential for vascular morphogenesis, but how NRP1 functions to guide vascular development has not been completely elucidated. In this study, we generated a mouse line harboring a point mutation in the endogenous Nrp1 locus that selectively abolishes VEGF-NRP1 binding (Nrp1VEGF−). Nrp1VEGF− mutants survive to adulthood with normal vasculature revealing that NRP1 functions independent of VEGF-NRP1 binding during developmental angiogenesis. Moreover, we found that Nrp1-deficient vessels have reduced VEGFR2 surface expression in vivo demonstrating that NRP1 regulates its co-receptor, VEGFR2. Given the resources invested in NRP1-targeted anti-angiogenesis therapies, our results will be integral for developing strategies to re-build vasculature in disease. DOI: http://dx.doi.org/10.7554/eLife.03720.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.03720en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197402/pdf/en
dash.licenseLAAen_US
dc.subjectNeuropilin-1en
dc.subjectdevelopmental angiogenesisen
dc.subjectVEGFR2en
dc.subjectVEGFen
dc.subjectmouse geneticsen
dc.subjectmouseen
dc.titleNeuropilin-1 functions as a VEGFR2 co-receptor to guide developmental angiogenesis independent of ligand bindingen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorTata, Aleksandraen_US
dc.date.available2014-11-03T17:39:54Z
dc.identifier.doi10.7554/eLife.03720*
dash.contributor.affiliatedLacoste, Baptiste
dash.contributor.affiliatedTata, Aleksandra
dash.contributor.affiliatedBischoff, Joyce
dash.contributor.affiliatedWang, Jia-Huai
dash.contributor.affiliatedGu, Chenghua


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