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dc.contributor.authorSadaka, Amaen_US
dc.contributor.authorPalmer, Kellien_US
dc.contributor.authorSuzuki, Takashien_US
dc.contributor.authorGilmore, Michael S.en_US
dc.date.accessioned2014-11-03T17:40:16Z
dc.date.issued2014en_US
dc.identifier.citationSadaka, Ama, Kelli Palmer, Takashi Suzuki, and Michael S. Gilmore. 2014. “In Vitro and In Vivo Models of Staphylococcus aureus Endophthalmitis Implicate Specific Nutrients in Ocular Infection.” PLoS ONE 9 (10): e110872. doi:10.1371/journal.pone.0110872. http://dx.doi.org/10.1371/journal.pone.0110872.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347593
dc.description.abstractPurpose To define global transcriptional responses of Staphylococcus aureus and its codY mutant (CodY is a transcription regulator of virulence and metabolic genes in response to branched-chain amino acids) when growing in bovine aqueous (AH) and vitreous humor (VH) in vitro, and to investigate the impact of codY deletion on S. aureus virulence in a novel murine anterior chamber (AC) infection model. Methods: For the in vitro model, differential transcriptomic gene expression of S. aureus and its codY mutant grown in chemically defined medium (CDM), AH, and VH was analyzed. Furthermore, the strains were inoculated into the AC of mice. Changes in bacterial growth, electroretinography and inflammation scores were monitored. Results: Bovine AH and VH provide sufficient nutrition for S. aureus growth in vitro. Transcriptome analysis identified 72 unique open reading frames differentially regulated ≥10-fold between CDM, AH, and VH. In the AC model, we found comparable growth of the codY mutant and wild type strains in vivo. Average inflammation scores and retinal function were significantly worse for codY mutant-infected eyes at 24 h post-infection. Conclusion: Our in vitro bovine AH and VH models identified likely nutrient sources for S. aureus in the ocular milieu. The in vivo model suggests that control of branched-chain amino acid availability has therapeutic potential in limiting S. aureus endophthalmitis severity.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0110872en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4207797/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectMicrobiologyen
dc.subjectMedical Microbiologyen
dc.subjectMicrobial Pathogensen
dc.subjectBacterial Pathogensen
dc.subjectStaphylococcusen
dc.subjectStaphylococcus Aureusen
dc.subjectMedicine and Health Sciencesen
dc.subjectOphthalmologyen
dc.titleIn Vitro and In Vivo Models of Staphylococcus aureus Endophthalmitis Implicate Specific Nutrients in Ocular Infectionen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorGilmore, Michael S.en_US
dc.date.available2014-11-03T17:40:16Z
dc.identifier.doi10.1371/journal.pone.0110872*
dash.contributor.affiliatedGilmore, Michael


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