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dc.contributor.authorSubtelny, Alexander O.en_US
dc.contributor.authorEichhorn, Stephen W.en_US
dc.contributor.authorChen, Grace R.en_US
dc.contributor.authorSive, Hazelen_US
dc.contributor.authorBartel, David P.en_US
dc.date.accessioned2014-11-03T17:40:27Z
dc.date.issued2014en_US
dc.identifier.citationSubtelny, Alexander O., Stephen W. Eichhorn, Grace R. Chen, Hazel Sive, and David P. Bartel. 2014. “Poly(A)-tail profiling reveals an embryonic switch in translational control.” Nature 508 (7494): 66-71. doi:10.1038/nature13007. http://dx.doi.org/10.1038/nature13007.en
dc.identifier.issn0028-0836en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347616
dc.description.abstractPoly(A) tails enhance the stability and translation of most eukaryotic mRNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here, we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measure tail lengths of millions of individual RNAs isolated from yeasts, cell lines, Arabidopsis leaves, mouse liver, and zebrafish and frog embryos. Poly(A)-tail lengths were conserved between orthologous mRNAs, with mRNAs encoding ribosomal proteins and other “housekeeping” proteins tending to have shorter tails. As expected, tail lengths were coupled to translational efficiency in early zebrafish and frog embryos. However, this strong coupling diminished at gastrulation and was absent in non-embryonic samples, indicating a rapid developmental switch in the nature of translational control. This switch complements an earlier switch to zygotic transcriptional control and explains why the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational repression to mRNA destabilization.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nature13007en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086860/pdf/en
dash.licenseLAAen_US
dc.subjectpoly(A)-tail lengthen
dc.subjecttranslational regulationen
dc.subjecttranslational efficiencyen
dc.subjectembryonic developmenten
dc.subjecthigh-throughput sequencingen
dc.subjectribosome profilingen
dc.titlePoly(A)-tail profiling reveals an embryonic switch in translational controlen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNatureen
dash.depositing.authorSubtelny, Alexander O.en_US
dc.date.available2014-11-03T17:40:27Z
dc.identifier.doi10.1038/nature13007*
dash.contributor.affiliatedSubtelny, Alexander


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