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dc.contributor.authorQuan, Jinhuaen_US
dc.contributor.authorAdelmant, Guillaumeen_US
dc.contributor.authorMarto, Jarrod A.en_US
dc.contributor.authorLook, A. Thomasen_US
dc.contributor.authorYusufzai, Timuren_US
dc.date.accessioned2014-11-03T17:40:36Z
dc.date.issued2014en_US
dc.identifier.citationQuan, Jinhua, Guillaume Adelmant, Jarrod A. Marto, A. Thomas Look, and Timur Yusufzai. 2014. “The Chromatin Remodeling Factor CHD5 Is a Transcriptional Repressor of WEE1.” PLoS ONE 9 (9): e108066. doi:10.1371/journal.pone.0108066. http://dx.doi.org/10.1371/journal.pone.0108066.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347631
dc.description.abstractLoss of the chromatin remodeling ATPase CHD5 has been linked to the progression of neuroblastoma tumors, yet the underlying mechanisms behind the tumor suppressor role of CHD5 are unknown. In this study, we purified the human CHD5 complex and found that CHD5 is a component of the full NuRD transcriptional repressor complex, which also contains methyl-CpG binding proteins and histone deacetylases. The CHD5/NuRD complex appears mutually exclusive with the related CHD4/NuRD complex as overexpression of CHD5 results in loss of the CHD4 protein in cells. Following a search for genes that are regulated by CHD5 in neuroblastoma cells, we found that CHD5 binds to and represses the G2/M checkpoint gene WEE1. Reintroduction of CHD5 into neuroblastoma cells represses WEE1 expression, demonstrating that CHD5 can function as a repressor in cells. A catalytically inactive mutant version of CHD5 is able to associate with a NuRD cofactor but fails to repress transcription. Our study shows that CHD5 is a NuRD-associated transcriptional repressor and identifies WEE1 as one of the CHD5-regulated genes that may link CHD5 to tumor suppression.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0108066en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172601/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and life sciencesen
dc.subjectBiochemistryen
dc.subjectProteinsen
dc.subjectDNA-binding proteinsen
dc.subjectCell Biologyen
dc.subjectChromosome Biologyen
dc.subjectChromosomesen
dc.subjectChromosome Structure and Functionen
dc.subjectChromatinen
dc.subjectGeneticsen
dc.subjectEpigeneticsen
dc.subjectHistone Modificationen
dc.subjectGene expressionen
dc.subjectDNA transcriptionen
dc.subjectGene regulationen
dc.titleThe Chromatin Remodeling Factor CHD5 Is a Transcriptional Repressor of WEE1en
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorQuan, Jinhuaen_US
dc.date.available2014-11-03T17:40:36Z
dc.identifier.doi10.1371/journal.pone.0108066*
dash.contributor.affiliatedQuan, Jinhua
dash.contributor.affiliatedAdelmant, Guillaume
dash.contributor.affiliatedYusufzai, Timur
dash.contributor.affiliatedMarto, Jarrod


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