Show simple item record

dc.contributor.authorHuang, Chaoen_US
dc.contributor.authorKaza, Aditya K.en_US
dc.contributor.authorHitchcock, Robert W.en_US
dc.contributor.authorSachse, Frank B.en_US
dc.date.accessioned2014-11-03T17:40:40Z
dc.date.issued2014en_US
dc.identifier.citationHuang, Chao, Aditya K. Kaza, Robert W. Hitchcock, and Frank B. Sachse. 2014. “Local delivery of fluorescent dye for fiber-optics confocal microscopy of the living heart.” Frontiers in Physiology 5 (1): 367. doi:10.3389/fphys.2014.00367. http://dx.doi.org/10.3389/fphys.2014.00367.en
dc.identifier.issn1664-042Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13347639
dc.description.abstractFiber-optics confocal microscopy (FCM) is an emerging imaging technology with various applications in basic research and clinical diagnosis. FCM allows for real-time in situ microscopy of tissue at sub-cellular scale. Recently FCM has been investigated for cardiac imaging, in particular, for discrimination of cardiac tissue during pediatric open-heart surgery. FCM relies on fluorescent dyes. The current clinical approach of dye delivery is based on systemic injection, which is associated with high dye consumption, and adverse clinical events. In this study, we investigated approaches for local dye delivery during FCM imaging based on dye carriers attached to the imaging probe. Using three-dimensional confocal microscopy, automated bench tests, and FCM imaging we quantitatively characterized dye release of carriers composed of open-pore foam only and foam loaded with agarose hydrogel. In addition, we compared local dye delivery with a model of systemic dye delivery in the isolated perfused rodent heart. We measured the signal-to-noise ratio (SNR) of images acquired in various regions of the heart. Our evaluations showed that foam-agarose dye carriers exhibited a prolonged dye release vs. foam-only carriers. Foam-agarose dye carriers allowed reliable imaging of 5–9 lines, which is comparable to 4–8 min of continuous dye release. Our study in the living heart revealed that the SNR of FCM images using local and systemic dye delivery is not different. However, we observed differences in the imaged tissue microstructure with the two approaches. Structural features characteristic of microvasculature were solely observed for systemic dye delivery. Our findings suggest that local dye delivery approach for FCM imaging constitutes an important alternative to systemic dye delivery. We suggest that the approach for local dye delivery will facilitate clinical translation of FCM, for instance, for FCM imaging during pediatric heart surgery.en
dc.language.isoen_USen
dc.publisherFrontiers Media S.A.en
dc.relation.isversionofdoi:10.3389/fphys.2014.00367en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174735/pdf/en
dash.licenseLAAen_US
dc.subjectfiber-optics confocal microscopyen
dc.subjectcardiac microscopyen
dc.subjectfluorescent dyesen
dc.subjectdye carrieren
dc.subjectcardiac surgeryen
dc.titleLocal delivery of fluorescent dye for fiber-optics confocal microscopy of the living hearten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalFrontiers in Physiologyen
dash.depositing.authorKaza, Aditya K.en_US
dc.date.available2014-11-03T17:40:40Z
dc.identifier.doi10.3389/fphys.2014.00367*
dash.contributor.affiliatedKaza, Aditya


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record