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dc.contributor.authorOh, S.-M.
dc.contributor.authorLiu, Z.
dc.contributor.authorOkada, M.
dc.contributor.authorJang, S.-W.
dc.contributor.authorLiu, X.
dc.contributor.authorChan, C.-B.
dc.contributor.authorLuo, Hongbo
dc.contributor.authorYe, K.
dc.date.accessioned2014-11-04T14:20:48Z
dc.date.issued2010
dc.identifier.citationOh, S.-M., Z. Liu, M. Okada, S.-W. Jang, X. Liu, C.-B. Chan, Hongbo Luo, and K. Ye. 2010. “Ebp1 Sumoylation, Regulated by TLS/FUS E3 Ligase, Is Required for Its Anti-Proliferative Activity.” Oncogene 29 (7) (November 30): 1017–1030. doi:10.1038/onc.2009.411. http://dx.doi.org/10.1038/onc.2009.411.en_US
dc.identifier.issn0950-9232en_US
dc.identifier.issn1476-5594en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13348318
dc.description.abstractEbp1, an ErbB3 receptor-binding protein, inhibits cell proliferation and acts as a putative tumor suppressor. Ebp1 translocates into the nucleus and functions as a transcription co-repressor for E2F-1. Here, we show that Ebp1 p42 isoform can be sumoylated on both K93 and K298 residues, which mediate its nuclear translocation and are required for its anti-proliferative activity. We find that translocation in liposarcoma (TLS)/FUS, an RNA-binding nuclear protein that is involved in pre-mRNA processing and nucleocytoplasmic shuttling, has Sumo1 E3 ligase activity for Ebp1 p42. Ebp1 directly binds TLS/FUS, which is regulated by genotoxic stress-triggered phosphorylation on Ebp1. Ebp1 sumoylation facilitates its nucleolar distribution and protein stability. Overexpression of TLS enhances Ebp1 sumoylation, whereas depletion of TLS abolishes Ebp1 sumoylation. Moreover, unsumoylated Ebp1 mutants fail to suppress E2F-1-regulated transcription, resulting in loss of its anti-proliferation activity. Hence, TLS-mediated sumoylation is required for Ebp1 transcriptional repressive activity.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/onc.2009.411en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/19946338en_US
dash.licenseMETA_ONLY
dc.subjectEbp1en_US
dc.subjectTLS/FUSen_US
dc.subjectsumoylationen_US
dc.subjectcell proliferationen_US
dc.titleEbp1 Sumoylation, Regulated by TLS/FUS E3 Ligase, Is Required for Its Anti-Proliferative Activityen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalOncogeneen_US
dash.depositing.authorLuo, Hongbo
dash.embargo.until10000-01-01
dc.identifier.doi10.1038/onc.2009.411*
dash.authorsorderedfalse
dash.contributor.affiliatedLuo, Hongbo


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