Inositol Hexakisphosphate Kinase 1 Regulates Neutrophil Function in Innate Immunity by Inhibiting Phosphatidylinositol-(3,4,5)-Trisphosphate Signaling

Prasad, Amit; Jia, Yonghui; Chakraborty, Anutosh; Li, Yitang; Jain, Supriya K.; Zhong, Jia; Roy, Saurabh Ghosh; Loison, Fabien; Mondal, Subhanjan; Sakai, Jiro; Blanchard, Catlyn; Snyder, Solomon H.; Luo, Hongbo

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Author

Prasad, Amit

Jia, Yonghui

Chakraborty, Anutosh

Li, Yitang

Jain, Supriya K.

Zhong, Jia

Roy, Saurabh Ghosh

Loison, Fabien HARVARD

Mondal, Subhanjan

Sakai, Jiro

Blanchard, Catlyn

Snyder, Solomon H.

Luo, Hongbo HARVARD

Note: Order does not necessarily reflect citation order of authors.

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https://doi.org/10.1038/ni.2052

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Citation

Prasad, Amit, Yonghui Jia, Anutosh Chakraborty, Yitang Li, Supriya K. Jain, Jia Zhong, Saurabh Ghosh Roy, et al. 2011. “Inositol Hexakisphosphate Kinase 1 Regulates Neutrophil Function in Innate Immunity by Inhibiting Phosphatidylinositol-(3,4,5)-Trisphosphate Signaling.” Nature Immunology 12 (8) (June 19): 752–760. doi:10.1038/ni.2052. http://dx.doi.org/10.1038/ni.2052.

Abstract

Inositol phosphates are widely produced throughout animal and plant tissues. Diphosphoinositol pentakisphosphate (InsP7) contains an energetic pyrophosphate bond. Here we demonstrate that disruption of inositol hexakisphosphate kinase 1 (InsP6K1), one of the three mammalian inositol hexakisphosphate kinases (InsP6Ks) that convert inositol hexakisphosphate (InsP6) to InsP7, conferred enhanced phosphatidylinositol-(3,4,5)-trisphosphate \((PtdIns(3,4,5)P_3)\)-mediated membrane translocation of the pleckstrin homology domain of the kinase Akt and thus augmented downstream \(PtdIns(3,4,5)P_3\) signaling in mouse neutrophils. Consequently, these neutrophils had greater phagocytic and bactericidal ability and amplified NADPH oxidase–mediated production of superoxide. These phenotypes were replicated in human primary neutrophils with pharmacologically inhibited InsP6Ks. In contrast, an increase in intracellular InsP7 blocked chemoattractant-elicited translocation of the pleckstrin homology domain to the membrane and substantially suppressed \(PtdIns(3,4,5)P_3\)-mediated cellular events in neutrophils. Our findings establish a role for InsP7 in signal transduction and provide a mechanism for modulating \(PtdIns(3,4,5)P_3\) signaling in neutrophils.