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dc.contributor.authorGifford, Casey A.
dc.contributor.authorZiller, Michael Johannes
dc.contributor.authorGu, Hongcang
dc.contributor.authorTrapnell, Cole
dc.contributor.authorDonaghey, Julie A
dc.contributor.authorTsankov, Alexander
dc.contributor.authorShalek, Alex K.
dc.contributor.authorKelley, David Roy
dc.contributor.authorShishkin, Alexander A.
dc.contributor.authorIssner, Robbyn
dc.contributor.authorZhang, Xiaolan
dc.contributor.authorCoyne, Michael J.
dc.contributor.authorFostel, Jennifer L.
dc.contributor.authorHolmes, Laurie
dc.contributor.authorMeldrim, Jim
dc.contributor.authorGuttman, Mitchell
dc.contributor.authorEpstein, Charles
dc.contributor.authorPark, Hongkun
dc.contributor.authorKohlbacher, Oliver
dc.contributor.authorRinn, John L
dc.contributor.authorGnirke, Andreas
dc.contributor.authorLander, Eric Steven
dc.contributor.authorBernstein, Bradley E.
dc.contributor.authorMeissner, Alexander
dc.date.accessioned2014-11-07T16:41:07Z
dc.date.issued2013
dc.identifier.citationGifford, Casey A., Michael J. Ziller, Hongcang Gu, Cole Trapnell, Julie Donaghey, Alexander Tsankov, Alex K. Shalek, et al. 2013. “Transcriptional and Epigenetic Dynamics During Specification of Human Embryonic Stem Cells.” Cell 153, no. 5: 1149–1163.en_US
dc.identifier.issn0092-8674en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13362672
dc.description.abstractDifferentiation of human embryonic stem cells (hESCs) provides a unique opportunity to study the regulatory mechanisms that facilitate cellular transitions in a human context. To that end, we performed comprehensive transcriptional and epigenetic profiling of populations derived through directed differentiation of hESCs representing each of the three embryonic germ layers. Integration of whole-genome bisulfite sequencing, chromatin immunoprecipitation sequencing, and RNA sequencing reveals unique events associated with specification toward each lineage. Lineage-specific dynamic alterations in DNA methylation and H3K4me1 are evident at putative distal regulatory elements that are frequently bound by pluripotency factors in the undifferentiated hESCs. In addition, we identified germ-layer-specific H3K27me3 enrichment at sites exhibiting high DNA methylation in the undifferentiated state. A better understanding of these initial specification events will facilitate identification of deficiencies in current approaches, leading to more faithful differentiation strategies as well as providing insights into the rewiring of human regulatory programs during cellular transitions.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionofdoi:10.1016/j.cell.2013.04.037en_US
dash.licenseMETA_ONLY
dc.titleTranscriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cellsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalCellen_US
dash.depositing.authorMeissner, Alexander
dash.embargo.until10000-01-01
dc.identifier.doi10.1016/j.cell.2013.04.037*
dash.authorsorderedfalse
dash.contributor.affiliatedCoyne, Michael
dash.contributor.affiliatedDonaghey, Julie
dash.contributor.affiliatedTsankov, Alexander M.
dash.contributor.affiliatedZiller, Michael
dash.contributor.affiliatedKelley, David Roy
dash.contributor.affiliatedRinn, John
dash.contributor.affiliatedBernstein, Bradley
dash.contributor.affiliatedLander, Eric
dash.contributor.affiliatedMeissner, Alexander
dash.contributor.affiliatedPark, Hongkun


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